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32,167 results

Showing: 8131 - 8160 of 32167 results

  1. 1257 pGEX4T3 GST FKHR

    Plasmid
    #10706
    Depositor
    William Sellers
    Insert
    FKHR (FOXO1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 14, 2006
    Availability
    Academic Institutions and Nonprofits only

  4. pJJS008

    Plasmid
    #188331
    Purpose
    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::wrmScarlet::mIAA7
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188331-plasmid-map-sequence-id-370747

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  6. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pBAD33.1-DpnII

    Plasmid
    #127413
    Purpose
    Expresses DpnII restriction endonuclease in bacteria
    Depositor
    Douglas Feldman
    Insert
    DpnII
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    127413-plasmid-map-sequence-id-247669

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  10. (SUMO)5-(SIM)10

    Plasmid
    #126923
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Expression
    Bacterial
    Mutation
    internal BamHI site between (SUMO)5 and (SIM)10
    Promoter
    tac
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126923-plasmid-map-sequence-id-255302

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  11. pCW17 - pAdapter

    Plasmid
    #174664
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    MS2 coat protein
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174664-plasmid-map-sequence-id-361957

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  14. pKB223

    Plasmid
    #170013
    Purpose
    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    C18G
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    170013-plasmid-map-sequence-id-335942

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  16. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  22. pNDGG050

    Plasmid
    #231319
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231319-plasmid-map-sequence-id-466017

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  27. MZB134

    Plasmid
    #228485
    Purpose
    "Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    6xHis-TEV-Halo-HRV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228485-plasmid-map-sequence-id-455390

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  28. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Stephen Benkovic
    Insert
    uvsY (uvsY Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228214-plasmid-map-sequence-id-455995

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Showing: 8131 - 8160 of 32167 results