30,296 results

1. pQE60 R124K wgMDH

   Plasmid

   #204258

   Purpose

   Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124K

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE60 R124Q wgMDH

   Plasmid

   #204259

   Purpose

   Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124Q

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE60 R124A wgMDH

   Plasmid

   #204257

   Purpose

   Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE60 R130E wgMDH

   Plasmid

   #204267

   Purpose

   Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130E

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAraGFPCDF

   Plasmid

   #47516

   Purpose

   Contains PBAD::GFP reporter, used for heterodimer competition assay

   Depositor

   Bryan Berger

   Article

   Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINB-M1-di-Ub(G76V)-HOIL-1

   Plasmid

   #229539

   Purpose

   Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli

   Depositor

   Bernhard Lechtenberg

   Article

   Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025

   Inserts

   Ubiquitin (UBC Human)
   Ubiquitin (UBC Human)
   HOIL-1 (RBCK1 Human)
   

   Use

   Tags

   6xHis-3C

   Expression

   Bacterial

   Mutation

   Changed Gly 76 to Val and changed Gly 76 to Val

   Promoter

   T7

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMJ923

   Plasmid

   #78313

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-mCherry-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BRCA1 (1-304)

   Plasmid

   #12645

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   E3-Ring domain, Ub-ligase (BRCA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p6187

   Plasmid

   #198158

   Purpose

   bacterial expression of sifA::HaloTag fusion, mutant form

   Depositor

   Michael Hensel

   Insert

   PsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   HaloTag-HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCPP5154

    Plasmid

    #128704

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    avrPto
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLA230

    Plasmid

    #11724

    Depositor

    Lawrence Loeb

    Article

    Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

    Insert

    b-lactamase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GAA for Glu26 changed to TAA (premature stop codo…

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p405TDH3

    Plasmid

    #15969

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    p405TDH3 (TDH3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    TDH3 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGS-21a-PcAad1

    Plasmid

    #163039

    Purpose

    expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

    Depositor

    Jean-Marie Francois

    Article

    Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

    Insert

    aryl-alcohol dehydrogenase
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMApra-Plac-SO

    Plasmid

    #218358

    Purpose

    Empty vector with lacIq-Plac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SUMO_TwinStrep_Cas7-11_crRNA

    Plasmid

    #197612

    Purpose

    For protein purification of Cas7-11

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    SUMO_TwinStrep_Cas7-11 with crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEXP5-NT/pLux eGFP

    Plasmid

    #193624

    Purpose

    Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    eGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEXP5-NT/pT7 LuxR

    Plasmid

    #193625

    Purpose

    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LuxR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KRASB

    Plasmid

    #25153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KRASb:M1-K169 (KRAS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q61H

    Promoter

    Available since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits