32,926 results
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Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBAD-Citrine2
Plasmid#110772PurposeYellow fluorescent protein with improved photostabilityDepositorInsertCitrine2
TagsHis tagExpressionBacterialAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2
Plasmid#124218PurposeCytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2DepositorInsertmNG-sfTq2
ExpressionBacterialAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR2B-dCas9-KRAB
Plasmid#115547PurposeEntry cassette for dCas9-KRABDepositorInsertdCas9-KRAB
TagsHAExpressionBacterial and MammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Strep2
Plasmid#119753PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7 C450A
Plasmid#104628Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutationDepositorInsertCaspase-3 (CASP3 Human)
TagsHis and LOV2ExpressionBacterialMutationC450A (dark-locked and light-insensitive mutation)PromoterT7Available SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15aC-GFP
Plasmid#107862PurposeControl plasmid expressing GFPDepositorInsertGFP
TagsmycExpressionBacterialPromoterproDAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D
Plasmid#107863PurposeBacterial expression of PISDepositorInsertPIS
TagsmycExpressionBacterialPromoterproDAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b_NOSER
Plasmid#124244PurposeBacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-iRFPBb
Plasmid#118237PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFPDepositorInsertflagellin promoter driven iRFP
ExpressionBacterialAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXS-sfGFP
Plasmid#117386PurposepGEN-mcs with a modular sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
TagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROP-IP-GFP
Plasmid#122136PurposeGFP Reporter plasmid for B-Rex contain imperfect operator sequenceDepositorInsertGFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
TagsHis6 Flag tagExpressionBacterialPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMal_Abl-PRM 3R
Plasmid#112086PurposeBacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-3R (ABL1 Human)
TagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-Abl-PRM 4R
Plasmid#112087PurposeBacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-4R (ABL1 Human)
TagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
ExpressionBacterialPromoterPtetAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
ExpressionBacterialPromoterE1b minimal promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_44
Plasmid#109242PurposepSC101 var2 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_47
Plasmid#109245PurposepSC101 var5 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only