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pET22b-ELP[V]60

Plasmid

#219406

Purpose

(VPGVG)60

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

ELP[V]60

Tags

6xHis

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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WT USP7 catalytic

Plasmid

#227201

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 207-560

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTSAR3.5t

Plasmid

#210257

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

eBFP2
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0283 (pTnsABC)

Plasmid

#130633

Purpose

Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Tags

3xHA

Expression

Bacterial and Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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