30,808 results

1. MP2

   Plasmid

   #69628

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926
   

   Expression

   Bacterial

   Promoter

   E. coli PBAD

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-X(5010)B1

   Plasmid

   #168019

   Purpose

   Expresses X(5010)B1-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   X(5010)B1-6xHis
   

   Tags

   Hisx6

   Expression

   Bacterial

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. DDX20A

   Plasmid

   #42376

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DDX20A (DDX20 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET-53-RtcA

   Plasmid

   #107876

   Purpose

   Expresses E. coli RtcA in bacterial expression cells

   Depositor

   Jay Hesselberth

   Insert

   E. coli RtcA (rtcA Escherichia coli)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYTK066

   Plasmid

   #65173

   Purpose

   Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   tTDH1
   

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

   Plasmid

   #159698

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYTK-DN5

   Plasmid

   #180286

   Purpose

   NatR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - NatR - CEN6/ARS4
   

   Expression

   Bacterial and Yeast

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRT498-OtUBD(V203D)

   Plasmid

   #190090

   Purpose

   Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD(V203D)
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUB7

   Plasmid

   #169698

   Purpose

   Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Grp1-PH pGEX2TK

    Plasmid

    #71379

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    tac

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. MBP-FUS_FL_6E

    Plasmid

    #98655

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    6 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p15aC-4D1D-5

    Plasmid

    #107866

    Purpose

    Bacterial expression of PIS, PI4K and PI4P5K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 4-kinase β
    

    Tags

    myc

    Expression

    Bacterial

    Promoter

    proD and proD (in operon)

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human HUWE1 (3993-4374)

    Plasmid

    #110754

    Purpose

    Bacterial Expression of HUWE1 HECT domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. t1-435

    Plasmid

    #166578

    Purpose

    For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

    Insert

    Tln1 Head (1-435) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-435 only

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MYH11-gRNA1

    Plasmid

    #132587

    Purpose

    gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    MYH11-gRNA1
    

    Expression

    Bacterial

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQLinkHD

    Plasmid

    #13668

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    Gateway cassette
    

    Use

    Co-expression

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAB152

    Plasmid

    #101663

    Purpose

    Opto-T7RNAP*(563-F2)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563-F2)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCmEos3.2:cat

    Plasmid

    #78100

    Purpose

    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

    Depositor

    Peng Chen

    Article

    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

    Insert

    mEos3.2-cat
    

    Expression

    Bacterial

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pReporter_12

    Plasmid

    #60572

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_12
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. NEDD4

    Plasmid

    #25141

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    nedd4.0001.0152 (NEDD4 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits