30,808 results

1. pBTK552

   Plasmid

   #110618

   Purpose

   BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pUdO4z

   Plasmid

   #210253

   Purpose

   low-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUdO2z

   Plasmid

   #210251

   Purpose

   medium-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUdO1c

   Plasmid

   #210230

   Purpose

   high-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-His tagged nuclear Pif1

   Plasmid

   #65047

   Purpose

   expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

   Depositor

   Jean-Baptiste Boulé, Virgina Zakian

   Article

   Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

   Insert

   Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
   

   Tags

   histag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-PHGDH

   Plasmid

   #74113

   Purpose

   Expression of N-terminal His-tagged

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   His-tagged

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSK+ chickBMPR-1B CA

   Plasmid

   #49528

   Purpose

   cloning vector for constitutively active chick BMP receptor 1B

   Depositor

   Lee Niswander, Peter ten Dijke

   Article

   Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

   Insert

   BMPR-1B CA (BMPR1B Chicken)
   

   Expression

   Bacterial

   Mutation

   constitutively active mutation (Q203D); also P2A,…

   Promoter

   T3

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pMH610

   Plasmid

   #131862

   Purpose

   Produces holo-PhyB* in E. coli

   Depositor

   Wilfried Weber

   Article

   Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

   Inserts

   PhyB(1-651)-RGD-His6-Cys
   HO1
   PcyA
   

   Expression

   Bacterial

   Promoter

   T7 promoter-1 and T7 promoter-2

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMyBADC

   Plasmid

   #84688

   Purpose

   Protein expression in mycobacteria

   Depositor

   Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Bacterial expression vector

   Tags

   His6

   Expression

   Bacterial

   Promoter

   Arabinose

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p405TDH3

    Plasmid

    #15969

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    p405TDH3 (TDH3 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    TDH3 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRedCm

    Plasmid

    #176225

    Purpose

    LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PrhaB and PL

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-MAT2A

    Plasmid

    #164820

    Purpose

    Expresses MAT2A in E. coli BL21(DE3)

    Depositor

    Vern Schramm

    Article

    Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

    Insert

    Methionine Adenosyltransferase 2A (MAT2A Human)
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KLHL7

    Plasmid

    #39023

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL7 (KLHL7 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCMV-Tag4A-MMP8 Wt

    Plasmid

    #29545

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. PtxS1-c003

    Plasmid

    #173076

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PtxS1
    

    Expression

    Bacterial

    Mutation

    D35-I221

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTrcHisB-TRF1

    Plasmid

    #53209

    Purpose

    Expressed N-terminally Hexahistidine tagged TRF1 protein

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF1 (TERF1 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    3-439

    Promoter

    Trc

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQ-NFY-A-GB-HC

    Plasmid

    #171081

    Purpose

    Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Inserts

    NF-YA (NFYA Human)
    NF-YB (NFYB Human)
    NF-YC (NFYC Human)
    

    Tags

    GST and His

    Expression

    Bacterial

    Promoter

    T5 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRE143

    Plasmid

    #118495

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge