30,808 results

1. RhoA

   Plasmid

   #55699

   Purpose

   expression of mouse RhoA

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RhoA (Rhoa Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Tags

   T120>A

   Expression

   Bacterial

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO GFP

   Plasmid

   #68488

   Purpose

   TOPO construct expressing GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Green Fluorescent Protein
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETa_ mEos4a

   Plasmid

   #51072

   Purpose

   Photoconverible Fluorescent Protein_ Fixative Resistant

   Depositor

   Loren Looger

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   mEos4a
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. His_Naa80d in pTYB12

   Plasmid

   #188455

   Purpose

   Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   Naa80 (NAA80 Human)
   

   Tags

   intein-CBD, 6xHis

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15p65

   Plasmid

   #27661

   Depositor

   Kathleen Collins

   Article

   Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

   Insert

   Tetrahymena telomerase holoenzyme p65
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pNIC28-Bsa4_ZV-NS5-RdRp

   Plasmid

   #179847

   Purpose

   The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

   Depositor

   Julien Lescar

   Article

   Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

   Insert

   Zika Virus Non-structural protein 5 RdRp domain
   

   Tags

   6-histine

   Expression

   Bacterial

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-AMVpl-δWT-nAchR

   Plasmid

   #97188

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Delta nicotinic acetylcholine receptor (Chrnd Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-p40PX(aa1-148)

   Plasmid

   #19008

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p40phox (NCF4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa1-148 (PX domains)

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

10. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. APneg-SNAP25a

    Plasmid

    #168011

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pV1F(Spec)

    Plasmid

    #205086

    Purpose

    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

    Depositor

    Suomeng Dong

    Article

    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

    Insert

    AtVIP1
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_hTLN2-FL

    Plasmid

    #164835

    Purpose

    Expression of FL human talin2

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Tags

    His8

    Expression

    Bacterial

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGST-PPARgamma

    Plasmid

    #16549

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Insert

    PPAR gamma (PPARG Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal DNA-binding domain: aa 1-248

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJM.683

    Plasmid

    #108531

    Purpose

    BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    BetIAM + PBetI-YFP
    

    Expression

    Bacterial

    Mutation

    BetI: S115I

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBPHLWL-VP16AD

    Plasmid

    #26259

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    VP16AD-ZIP+
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMO719

    Plasmid

    #117479

    Purpose

    Desulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specR

    Depositor

    Judy Wall

    Article

    Keller et al Appl Environ Microbiol. 2009 Dec;75(24):

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Tags

    Polyhistidine

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

    Plasmid

    #48307

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis(TEV)-Drp1-StrepII

    Plasmid

    #174422

    Purpose

    Expresses human Drp1 Isoform 3 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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