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pSMART-HCKan-yibDp-VHH-VCAM1

Plasmid

#202471

Purpose

Expresses the nanobody VCAM1 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VCAM1

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pKLxR5

Plasmid

#149465

Purpose

Broad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET303-colE1T

Plasmid

#155180

Purpose

Expresses colicin E1 residues 1-190

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-190

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-190

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHV739

Plasmid

#117477

Purpose

Expression of NMT2

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAN1717

Plasmid

#74696

Purpose

RPU standard plasmid

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Insulated J23101-YFP

Expression

Bacterial

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-trc- YFP

Plasmid

#186569

Purpose

p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

trc promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Cis1-SH2

Plasmid

#46417

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cis1 (SOCS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 90-214

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pNM58

Plasmid

#128409

Purpose

System with AIP and xylose inducible agrCA sensor

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Inserts

argCA
gfpmut2

Expression

Bacterial

Promoter

P3 and PxylR

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBSII(KSplus)-HsBGG-WT_E

Plasmid

#146187

Purpose

Bacterial Expression of HsBGG-WT

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

HsBGG-WT

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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RCP83

Plasmid

#163466

Purpose

Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

Depositor

Frank Albert

Article

Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

Inserts

Promoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP

Expression

Bacterial and Yeast

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT25

Plasmid

#89664

Purpose

expression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT25

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT19

Plasmid

#89661

Purpose

expression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT19

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLMB509

Plasmid

#40084

Depositor

Philip Poole

Article

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gfpmut 3.1
tauR
tau promoter

Expression

Bacterial

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPSU60

Plasmid

#173833

Purpose

Expresses 60 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPACRCC1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-21a_FnCpf1_2xNLS_protein_expression

Plasmid

#124548

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnCpf1_2xNLS

Use

CRISPR

Expression

Bacterial

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

Plasmid

#115207

Purpose

Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

BzoCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_RRMs

Plasmid

#157864

Purpose

express His tagged hnRNPA2 RRMs

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His

Expression

Bacterial

Mutation

190stop

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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23 pCOLI_A_N-SUMO_C_STREP

Plasmid

#160259

Purpose

N-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII, 6xHis, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

Plasmid

#159696

Purpose

E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)

Tags

His6-Halotag-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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