Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

33,449 results

Showing: 6241 - 6260 of 33449 results

  1. pET28a-TGFα-GSY-SPM

    Plasmid
    #163615
    Purpose
    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.
    Depositor
    Mark Howarth
    Publication
    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:
    Insert
    TGFa-GSY-SPM (TGFA Synthetic, Human)
    Tags
    C-tag and His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    163615-plasmid-map-sequence-id-323634

    Enlarge

  2. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112558-plasmid-map-sequence-id-217235

    Enlarge

  3. pAC-crRNA-Cm

    Plasmid
    #158711
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Publication
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    eforCP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    158711-plasmid-map-sequence-id-325466

    Enlarge

  4. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Publication
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

    Enlarge

  6. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Publication
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

    Enlarge

  9. mem-mCherry-pTRIP

    Plasmid
    #163521
    Purpose
    Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells
    Depositor
    Thomas Weber
    Insert
    membrane targeted mCherry
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163521-plasmid-map-sequence-id-321568

    Enlarge

  12. 6NM4

    Plasmid
    #162257
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM9 (PRDM9 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    162257-plasmid-map-sequence-id-319826

    Enlarge

Showing: 6241 - 6260 of 33449 results