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pBHRSF184

Plasmid

#89482

Purpose

Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Tags

Smt3

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Sox10

Plasmid

#46536

Purpose

A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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PJQ48

Plasmid

#134647

Purpose

Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23114

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

Plasmid

#55209

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xAsn, 10xHis, MPB, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTF-lacZ

Plasmid

#153036

Purpose

For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEEK1

Plasmid

#83488

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Kukil et al Microb Cell Fact. 2022 Jan 10;21(1):8. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Promoter

Ptrc2O

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_5Y33

Plasmid

#234204

Purpose

Heterologous protein expression of alginate lyase in Escherichia coli

Depositor

Christopher Bahl

Insert

5Y33

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCgVchCAST-crtYf

Plasmid

#203816

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBbS18k-RFP

Plasmid

#126197

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala1

Plasmid

#65814

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Insulated waaHp from E. coli

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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