30,320 results

1. pET-NtLNtC60αESC60β

   Plasmid

   #160875

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, groES
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcL: pBAD, chaperones: T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEB2_mScarletI_mVenusNB-MrkH

   Plasmid

   #182291

   Purpose

   expresses c-di-GMP biosensor in E. coli

   Depositor

   Howard Berg

   Article

   Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

   Insert

   mVenusNB-MrkH
   

   Use

   Tags

   mVenusNB internal fusion to MrkH

   Expression

   Bacterial

   Mutation

   Promoter

   proC

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET11a_PTK2b_Kinase

   Plasmid

   #224242

   Purpose

   Expression of human PTK2b Kinase domain in E. coli

   Depositor

   Andrew Mesecar

   Insert

   ptk2b_kinase (PTK2B Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. USP15

   Plasmid

   #28282

   Depositor

   Cheryl Arrowsmith

   Insert

   USP15 (USP15 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Contains dusp and Ubl domains (residues 6-223).

   Promoter

   Available since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

5. Ec2Avs2

   Plasmid

   #188790

   Purpose

   Avs2 from Escherichia coli NCTC10650

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Ec2Avs2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINB-hRNF216(511-784)

   Plasmid

   #171923

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-GGG-ELP(120nm)-Cys

   Plasmid

   #91572

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(120nm)-Cys
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX6P1-hPREP

   Plasmid

   #179387

   Purpose

   Express human prolyl oligopeptidase (tagged) in bacterial cells

   Depositor

   Christian Gruber

   Article

   Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

   Insert

   human prolyl oligopeptidase (PREP Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103_VDR_Hormone-recep

   Plasmid

   #109962

   Purpose

   Protein expression and purification of VDR_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   VDR_Hormone-recep
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSNAP-CLC-EYFP

    Plasmid

    #38011

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP and SNAP26m

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM6-G6-vioABECD

    Plasmid

    #66536

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLIPrbs

    Plasmid

    #17860

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    D208G

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMP220

    Plasmid

    #185921

    Purpose

    Broad host range IncP promoter probe using lacZ

    Depositor

    Herman Spaink

    Article

    Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi

    Type

    Empty backbone

    Use

    Incp

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ubiquitin K63C

    Plasmid

    #86589

    Purpose

    Bacterial expression of Ubiquitin K63C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K63C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pHG6

    Plasmid

    #188254

    Purpose

    Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKB817 (pBra-Hfq)

    Plasmid

    #174661

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

    Depositor

    Ann Hochschild, Katherine Berry

    Article

    Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

    Insert

    Hfq
    

    Use

    Tags

    alpha NTD

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid

    #90473

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Insert

    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMVV207

    Plasmid

    #215343

    Purpose

    gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    S. stutzeri nifL gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMVV205

    Plasmid

    #215342

    Purpose

    Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-His-APEX2

    Plasmid

    #201471

    Purpose

    Recombinant soluble APEX2 for SLAPSHOT labeling

    Depositor

    Yuh-Nung Jan

    Article

    Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

    Insert

    APEX2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge