32,838 results
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Plasmid#159422PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66DepositorInsertSb66
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/X
Plasmid#168017PurposeExpresses BoNT/X-LC(1-439)-6xHis in bacterial cellsDepositorInsertBoNT/X-LC(1-439)-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-mF(3230)A3
Plasmid#168016PurposeExpresses MBP-F(3230)A3-6xHis in bacterial cellsDepositorInsertMBP-F(3230)A3-6xHis
TagsHisx6 and maltose binding proteinExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_hTLN2-FL
Plasmid#164835PurposeExpression of FL human talin2DepositorAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Z-AIMP1-1
Plasmid#166560PurposescFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Q-IL18-6
Plasmid#166554PurposescFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-DARS-1
Plasmid#166538PurposescFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-mCerulean3-pBluescript
Plasmid#166757PurposeFor purification of mCerulean3 fluorophoreDepositorInsertmCerulean3
Tags6x Histidine tag, and TEV protease siteExpressionBacterialPromoterT7Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-Venus-pBluescript
Plasmid#166756PurposeFor purification of Venus fluorophoreDepositorInsertVenus
Tags6x Histidine tag, and TEV protease siteExpressionBacterialPromoterT7Available SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-LARS-15
Plasmid#166542PurposescFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
ExpressionBacterialPromoterPBADAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5588
Plasmid#128708PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopD1
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5053
Plasmid#128707PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopC1
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc090
Plasmid#161937PurposeCarries engineered transposon for IBMDepositorInsertpTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
ExpressionBacterialAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
TagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-6AlanineHE1
Plasmid#165098Purposebacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1DepositorAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-BR1
Plasmid#160333PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.DepositorInsertBR1
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPcR5
Plasmid#149484PurposeBroad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertPcaU(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterP3B5BAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCyR6
Plasmid#149493PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCyR5
Plasmid#149463PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLlR5
Plasmid#149464PurposeBroad-Host range expression vector, RK2 and pUC origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRR5
Plasmid#149468PurposeBroad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertRhaS-RhaR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCiG2
Plasmid#149470PurposeBroad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertCinR(AM)-GFP
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
RBFOX3_RRM_pGEX
Plasmid#135131PurposeRecombinant expression of RBFOX3 with dual-affinity tagDepositorAvailable SinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b
Plasmid#164862PurposeInducible expression of BzCas13b nuclease in bacteria.DepositorInsertBergeyella zoohelcum Cas13b
ExpressionBacterialPromoterT7 PromoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-NT
Plasmid#164861PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-NT
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
Tags6XHis and Tat(48-57)ExpressionBacterialAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only