34,237 results

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COC053

Plasmid

#209131

Purpose

Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

Depositor

Christopher A. O'Callaghan

Publication

O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

Insert

Enzyme BirA (birA E. coli)

Expression

Bacterial

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-LDh-LY-P2A

Plasmid

#214895

Purpose

Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites

Depositor

Tito Calì

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SPLICSFAST-LDh-LY-P2A

Tags

none

Expression

Bacterial and Mammalian

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSAP

Plasmid

#206429

Purpose

A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

Depositor

Bogumil Karas

Publication

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACRISPR-KM

Plasmid

#203587

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Publication

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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WIW_Scaffold2

Plasmid

#205574

Purpose

Nanobody that inhibits seeding of tau fibrils

Depositor

David Eisenberg

Publication

Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

WIW_Scaffold2

Tags

6X His

Expression

Bacterial

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUCXMG

Plasmid

#204409

Purpose

Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

Depositor

David Ackerley

Publication

Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

tac

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1037

Plasmid

#208736

Purpose

Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Publication

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PSarA1

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1038

Plasmid

#208737

Purpose

Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Publication

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PCap

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCap

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204817

Purpose

Bacterial expresison of Enterovirus D68 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV D68 3C protease

Expression

Bacterial

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Publication

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Publication

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-HygTK

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-msfGFP(V206K)-GBI

Plasmid

#205163

Purpose

express tdMCP-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Publication

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdMCP-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSW003-PpsbA-mNeonGreen

Plasmid

#205019

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-CreiLOV

Plasmid

#192817

Purpose

Expresses CreiLOV in bacteria

Depositor

Kiryl Piatkevich

Publication

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Expression

Bacterial

Promoter

araBAD promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Clomeleon

Plasmid

#203723

Purpose

A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

Depositor

George Augustine

Publication

Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cyan fluorescent protein linked yellow florescent protein

Expression

Bacterial and Mammalian

Mutation

Cyan Fluorescent Protein, Topaz Fluorescent Prote…

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PJEBAN4

Plasmid

#203683

Purpose

Expresses red fluorescent marker HcRed in Listeria monocytogenes

Depositor

Tine Rask Licht

Publication

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

HcRed

Expression

Bacterial

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Publication

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1a

Plasmid

#205304

Purpose

Expression of WHaloCaMP1a in E. coli

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a

Expression

Bacterial

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Publication

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Publication

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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