30,416 results

1. pMAL-his-LbCpf1-EC

   Plasmid

   #79008

   Purpose

   Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   LbCpf1
   

   Use

   Tags

   MBP-6xHis-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Use

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYHY1

   Plasmid

   #215926

   Purpose

   E. coli-Bacteroides shuttle vector.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC-CTHF

   Plasmid

   #26105

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   TEV - His6 - Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-mCherry

   Plasmid

   #66534

   Purpose

   Expresses mCherry under the control of the Concensus T7 Promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Consensus T7

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHMattR3-attR4-GFP

   Plasmid

   #196247

   Purpose

   pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL1521 (pSPIN, pSC101* backbone)

   Plasmid

   #160729

   Purpose

   Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPlacPbuCas13b-gRNA-T-deGFP

   Plasmid

   #184840

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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