30,327 results

1. Tus pGST-//

   Plasmid

   #53305

   Purpose

   Expresses GST-tagged E.coli replication terminator protein Tus.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   E.Coli Tus replication terminator protein
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL4369 (pEffector_TnpB2,ωRNA)

   Plasmid

   #210871

   Purpose

   Expresses GstTnpB2 and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstTnpB2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR47S Cm

   Plasmid

   #41892

   Depositor

   Howard Steinman

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMAL-c2E

   Plasmid

   #75291

   Purpose

   Expresses proteins in the cytoplasm as fusions to maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAraTMwt

   Plasmid

   #47514

   Purpose

   Contains A2B L980A TMCY mutant, expresses wt AraC for heterodimer competition assay

   Depositor

   Bryan Berger

   Article

   Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

   Insert

   Integrin alpha2B L980A TM-CYTO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLP-pR-himar-apraR

   Plasmid

   #199996

   Purpose

   Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Landing Pads
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRKO

   Plasmid

   #49456

   Purpose

   loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

   Depositor

   Marc Gartenberg

   Article

   Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

   Insert

   loxP-kanMX-loxP cassette
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKM263-ScPPX

   Plasmid

   #38327

   Depositor

   Florian Freimoser

   Article

   Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   GST, His, and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MacroH2A.2-c002

   Plasmid

   #173089

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROH2A2 (MACROH2A2 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K167-K372

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MacroH2A1.2-c002

    Plasmid

    #173088

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Q162-N372, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET47b-T7-AsCas12f-WT

    Plasmid

    #204640

    Purpose

    Expression of wild-type AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-his6-HA3-SNAPf

    Plasmid

    #188941

    Purpose

    T7 expression vector for making his6- and HA3-tagged SNAP protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-SNAPf
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJS008

    Plasmid

    #188331

    Purpose

    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::wrmScarlet::mIAA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET FLII12Pglu-700uDelta6

    Plasmid

    #13568

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid

    #139787

    Purpose

    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman, Jeannie Lee

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD_IPF2.0

    Plasmid

    #163124

    Purpose

    A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    IFP2.0
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

    Plasmid

    #109995

    Purpose

    Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMVA2

    Plasmid

    #119951

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFPV25.1_rpsM_mCerulean3

    Plasmid

    #124904

    Purpose

    rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    rpsM promoter and mCerulean3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEXP5-aHL_spytag_his

    Plasmid

    #107334

    Purpose

    Monomeric SpyTag-6×-His alpha-hemolysin

    Depositor

    George Church

    Article

    Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    alpha-hemolysin-SpyTag-6xHis
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge