30,808 results

1. HisTEV-cDAXX

   Plasmid

   #197012

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   DAXX
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. His Puc (aa 1-393)

   Plasmid

   #52295

   Purpose

   Bacterial expression of His tagged Drosophila Puc fragment

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   Puc (puc Fly)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pET-gSTEP1

   Plasmid

   #159376

   Purpose

   Expresses gSTEP1 in E. coli

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   gSTEP1
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL14

   Plasmid

   #106901

   Purpose

   pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   mutS
   lacI
   rhaR
   rhaS
   

   Expression

   Bacterial

   Mutation

   Lysine 622 was replaced by Alanine

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. [pSK104] pET28 SLC38A9N-His

   Plasmid

   #136142

   Purpose

   bacterial expression of SLC38A9 N term fragment (1-119)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   SLC38A9 N term fragment (1-119) (SLC38A9 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dlx5

   Plasmid

   #15538

   Depositor

   John Rubenstein

   Article

   Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

   Insert

   Dlx5 (Dlx5 Mouse)
   

   Expression

   Bacterial

   Available Since

   Aug. 20, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pTEV5_ RpMatB_2

   Plasmid

   #78206

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A S170A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A S170A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMO746

   Plasmid

   #117480

   Purpose

   upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

   Depositor

   Judy Wall

   Article

   Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

   Insert

   uracil phosphoribosyltransferase
   

   Expression

   Bacterial

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINVH

    Plasmid

    #26041

    Depositor

    Ray Owens

    Article

    Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

    Insert

    u-LacZ-KHHHHHH
    

    Tags

    KHHHHHH and u-phosphatase secretion leader

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid

    #114069

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pVvanCC

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPro33

    Plasmid

    #17810

    Depositor

    Jay Keasling

    Article

    Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. wt 6XHis-SNX9 pET15b

    Plasmid

    #34690

    Depositor

    Sandra Schmid

    Article

    Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

    Insert

    Sorting nexin 9
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pDIV537

    Plasmid

    #177704

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBSM

    Plasmid

    #67505

    Purpose

    Express the tRNAMet scaffold

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNAMet
    

    Expression

    Bacterial

    Promoter

    lpp

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pQTEV-RPL9

    Plasmid

    #31334

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ribosomal protein L9 (RPL9 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YWHAQ

    Plasmid

    #38931

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAQ (YWHAQ Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only