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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQStrep2-CRYM

Plasmid

#31293

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

crystallin, mu (CRYM Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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YWHAE

Plasmid

#39146

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

YWHAE (YWHAE Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pQC

Plasmid

#48100

Purpose

Cumate-inducible expression of ECFP-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and ECFP

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-133

Plasmid

#118519

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45

Plasmid

#126878

Purpose

Expresses human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

CDC45 (CDC45 Human)

Tags

No tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag WDR5

Plasmid

#15553

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pMYO

Plasmid

#34626

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized

Promoter

lpp

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pZa pBAD csgA CBDcex pLac Ara csgGEF

Plasmid

#226378

Purpose

Plasmid carries csgA and csgG,E,F genes of curli operon

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

csgA and csgG,E,F genes of curli operon

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

Plasmid

#122083

Purpose

Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-LaminB1

Use

Retroviral

Tags

V5 tag on Nter of LaminB1

Expression

Bacterial and Mammalian

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pETconNK

Plasmid

#81169

Purpose

pETconNK Empty Backbone

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Type

Empty backbone

Tags

c-myc

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-GFP

Plasmid

#174617

Purpose

Bacterial expression of GFP.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLacI

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AK2

Plasmid

#38829

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AK2 (AK2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pMC059-HisMS2_PLP_NucPhos_pac

Plasmid

#155039

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

Depositor

Paul Freemont

Article

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available Since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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NUDT3A

Plasmid

#42348

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NUDT3A (NUDT3 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pQLink-GST-4xCTD

Plasmid

#138471

Purpose

Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

4x CTD repeat

Promoter

T5

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAJT194

Plasmid

#202293

Purpose

Type IIS cloning plasmid for LPattB5 integrations w/ OriT

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pNO183

Plasmid

#101068

Purpose

ppsR2-bphP1 bicistronic operon plasmid

Depositor

Jeffrey Tabor

Article

Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

Inserts

ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
bphP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

insert guanine at position 430 to fix frameshift …

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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