30,808 results

1. pETM6-G6-vioABECD

   Plasmid

   #66536

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Mutant T7 Promoter - G6

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 2xNLS-pMJ915v2

   Plasmid

   #88916

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJCC_039 FnCas12a

   Plasmid

   #171666

   Purpose

   For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a
   

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28 E2 LBD-Tev-E1a-His

   Plasmid

   #232117

   Purpose

   For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   DBT (DBT Human)
   

   Tags

   6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETDuet-1 Flag-LplA

   Plasmid

   #232118

   Purpose

   For bacterial expression of Flag-tagged E. coli LplA.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   lplA (lplA Escherichia coli (strain K12))
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28 His-MBP-TEV-BAP-Human BCKDK

   Plasmid

   #232116

   Purpose

   For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (BCKDK Human)
   

   Tags

   6 x His, Biotin acceptor peptide (BAP), Maltose-B…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTW357bN

   Plasmid

   #227932

   Purpose

   Accessory plasmid for negative selection

   Depositor

   Tina Wang

   Article

   Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

   Inserts

   gIII-neg
   T7c
   

   Expression

   Bacterial

   Mutation

   R632S

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTW357d5

   Plasmid

   #227931

   Purpose

   High-stringency accessory plasmid for selection

   Depositor

   Tina Wang

   Article

   Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

   Inserts

   gIII (recoded)
   T7c
   

   Expression

   Bacterial

   Mutation

   R632S, Q649S

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAVS1_Cas9-hGem_gRNA2

   Plasmid

   #217659

   Purpose

   expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA2 targeting the human AAVS1 safe harbour locus
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBad33_ISAs26_reRNA

    Plasmid

    #205326

    Purpose

    Bacterial expression of ISAs26 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISAs26 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHH0103_NR1H3_Hormone-recep

    Plasmid

    #109950

    Purpose

    Protein expression and purification of NR1H3_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1H3_Hormone-recep
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6975 pPROEX-HTb-SIS1

    Plasmid

    #1233

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    sis1 (SIS1 Budding Yeast)
    

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pGEX6P1-optimizedNedd4.2d

    Plasmid

    #165103

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

    Insert

    Nedd4.2d (NEDD4L Human)
    

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MP5

    Plasmid

    #69677

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA ugi CDA1
    

    Expression

    Bacterial

    Promoter

    E. coli PBAD

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SUV39H2

    Plasmid

    #25115

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SUV39H2_04 (SUV39H2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pMS12: pDonor(AvCAST)

    Plasmid

    #168145

    Purpose

    AvCAST donor harboring non-functional cargo.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST right end (RE)
    AvCAST left end (LE)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SNRPA_Full-length_pGEX

    Plasmid

    #135134

    Purpose

    Recombinant expression of SNRPA with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SNRPA (SNRPA Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PP-GSK3

    Plasmid

    #25133

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    cgd4_240:C29838
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pMRE164

    Plasmid

    #118512

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbB8k-csg-amylase

    Plasmid

    #166859

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge