30,327 results

1. hSDH5_C83D

   Plasmid

   #113048

   Purpose

   Expression of mutant hSDH5

   Depositor

   Edward A. Berry

   Article

   Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

   Insert

   hSDH5_C83D (SDHAF2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C83D, Insert begins at amino acid 39

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MDO1-c014

   Plasmid

   #173103

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   S58-A325, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK131

   Plasmid

   #71548

   Purpose

   Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   Alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

   Plasmid

   #122085

   Purpose

   Express M.EcoGII fused to CENPC with destabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of CENPC

   Expression

   Bacterial and Mammalian

   Mutation

   L341F, R930L (see comments below)

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. NUDT1

   Plasmid

   #74660

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT1 (NUDT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pMS29: pDonor(PmcCAST)

   Plasmid

   #168162

   Purpose

   PmcCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST right end (RE)
   PmcCAST left end (LE)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUS252y

   Plasmid

   #191834

   Purpose

   Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.

   Depositor

   Nicholas Coleman

   Insert

   fuYFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET15b LFn-Fla

   Plasmid

   #84871

   Purpose

   Bacterial expression of LFn-Fla fusion

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBS_flGAD

   Plasmid

   #87031

   Purpose

   Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria

   Depositor

   Janice Blum

   Insert

   flGAD (GAD2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac-Z

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Use

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-5X1 Flag RbBP5

    Plasmid

    #15551

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    RbBP5 (RBBP5 Human)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pJV0042

    Plasmid

    #205138

    Purpose

    Expression of 6xHis-EGFP-SnxA in bacteria

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    SnxA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUMO-β′-COPI-WD40

    Plasmid

    #180753

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    β′-COPI-WD40 (SEC27 Budding Yeast)
    

    Use

    Tags

    N-terminal strep tag, His-tag, and SUMO tag

    Expression

    Bacterial

    Mutation

    Encodes residues 1-301; deleted 302-889; mutated …

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMal-Halo

    Plasmid

    #209349

    Purpose

    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagII

    Depositor

    Lucia Strader

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSW003-PpsbA-mNeonGreen

    Plasmid

    #205019

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINTF

    Plasmid

    #41114

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB1-coSCFP3A

    Plasmid

    #104015

    Purpose

    Plasmid encoding coSCFP3A

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    coSCFP3A
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Same protein sequence as SCFP3A (codon optimized …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 819 pGEX2T PTEN C124S

    Plasmid

    #10745

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C124S

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pHLU

    Plasmid

    #64167

    Purpose

    harbours marker genes HIS3, LEU2 and URA3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIII

    Plasmid

    #134355

    Purpose

    Phagemid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    CroAct3 63aa
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge