30,327 results

1. pColdII-SIRPT1-Bubalus bubalis

   Plasmid

   #75350

   Purpose

   Nt His-tag Bubalus bubalis Sacsin 86-1364

   Depositor

   Kalle Gehring

   Insert

   Sacsin Bubalus bubalis 86-1364
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_Nsp9

   Plasmid

   #156478

   Purpose

   Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

   Plasmid

   #171587

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS 6His

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   MKIEE-NLS-RfxCas13d-NLS-HA-6His
   

   Use

   Tags

   HA-6HIS and MKIEE

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIntegrase_7

   Plasmid

   #60578

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAH25-SceI

   Plasmid

   #129389

   Purpose

   Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Chloramphenicol acetyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   CAT promoter

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE1062

   Plasmid

   #172356

   Purpose

   Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

   Depositor

   Eriko Takano

   Article

   Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

   Inserts

   scbR (SCO6265 )
   kasOp
   

   Use

   Synthetic Biology; Integrative into streptomyces …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptRNA100

   Plasmid

   #154133

   Purpose

   Expresses a set of tRNAs in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   tRNAs operon under control of Ptac promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAP24

   Plasmid

   #70190

   Purpose

   Modular vector for secreted luciferase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   sLucopt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSFlp

    Plasmid

    #45470

    Depositor

    Michele Swanson

    Article

    Bryan et al Appl Environ Microbiol. 2011 Apr;77(7):2

    Insert

    Flp recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBL132

    Plasmid

    #104333

    Purpose

    Expresses taz from a LacI regulated promoter under inducible IPTG control.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    Taz
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtacI

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Use

    Tags

    G protein and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pJJS006

    Plasmid

    #188329

    Purpose

    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mNeonGreen::mIAA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MKK2

    Plasmid

    #29580

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    MKK2 (MAP2K2 Human)
    

    Use

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Constitutively active mutant; Starting Met remove…

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-ATG8(414)/GFP-AUT7(414)

    Plasmid

    #49424

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

    Depositor

    Daniel Klionsky

    Article

    Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pUltra-FPheKRS

    Plasmid

    #136631

    Purpose

    Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    FPheKRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac1 promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJTM-T7-mNeongreen-6xHis

    Plasmid

    #210868

    Purpose

    Plasmid used for expressing his--tagged mNeongreen for protein purification.

    Depositor

    Richard Murray

    Article

    Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

    Insert

    mNeongreen-6xHistag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge