-
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pET28-iLOV-Rsn2
Plasmid#61687Purposebacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOVDepositorInsertRanaspumin-2
UseTagsHis and iLOVExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD18-Gm
Plasmid#65100Purposebacterial expression vector with Gentamycin selection, based on pBAD18DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL2-IncB-GFP11x7
Plasmid#113031PurposeExpresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoterDepositorInsertIncB (incB Chlamydia trachomatis)
UseTagsGFP11x7 and flagExpressionBacterialMutationPromotertet inducibleAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB2-eGFP-6xHis
Plasmid#130982PurposeBacterial expression of mouse EB2-GFP-6xHis for protein purification.DepositorInsertEB2-GFP-6xHis (Mapre2 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0263
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR358.4
Plasmid#125114PurposeExpression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121DepositorInsertsnarL(REC)-liaR(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c010
Plasmid#173100PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP14 (PARP14 Human)
UseTagsmCeruleanExpressionBacterialMutationF1208-G1388PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MP-K12
Plasmid#69656PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA dinB
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJJH1260
Plasmid#36913DepositorInsertsScURA3
ScURA3
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBCiG2
Plasmid#149470PurposeBroad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertCinR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK033
Plasmid#65140PurposeEncodes Venus as a Type 3 part to be used in the Dueber YTK systemDepositorInsertVenus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092677
Plasmid#153883PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stopDepositorInsertfragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationwt;handful of SNPs as watermarksPromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pATetO 6XHis-MMLV RT
Plasmid#178797PurposeInducible expression of MMLV RT in E. coliDepositorInsertMMLV RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only