30,810 results

1. pGEX4T-HEPN

   Plasmid

   #75296

   Purpose

   Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

   Insert

   Human Sacsin 4441-4579
   

   Tags

   GST-Tag

   Expression

   Bacterial

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZE31-DsRed-ms2x2

   Plasmid

   #26248

   Depositor

   Philippe Cluzel

   Article

   Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

   Insert

   ms2 binding sites (x2)
   

   Expression

   Bacterial

   Available Since

   Oct. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Ki67 (LR)-mNeonGreen-IRESpuro2

   Plasmid

   #183743

   Purpose

   Expresses Ki67 (LR)-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   LR domain of Ki67

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_DNAJB1_DNAJ

   Plasmid

   #109918

   Purpose

   Protein expression and purification of DNAJB1_DNAJ

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   DNAJB1_DNAJ
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD_IPF2.0

   Plasmid

   #163124

   Purpose

   A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   IFP2.0
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. H6-auxilin

   Plasmid

   #62571

   Purpose

   6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

   Depositor

   Ernst Ungewickell

   Article

   Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

   Insert

   Auxilin
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   *see comment below

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

8. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-His6-UBCH7

   Plasmid

   #212711

   Purpose

   Bacterial expression of His6-UBCH7

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   UBCH7
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCH1 - pBait-empty

    Plasmid

    #174663

    Purpose

    arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-crRNA-Cm

    Plasmid

    #158711

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    eforCP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    TetO

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS415-SPT6

    Plasmid

    #10993

    Depositor

    Francisco Malagon

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Available Since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 5T5G (SETD8)

    Plasmid

    #101886

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD8 (KMT5A Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pJYP1

    Plasmid

    #202406

    Purpose

    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

    Depositor

    Remy Loris

    Article

    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    fdeA

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

    Plasmid

    #153524

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#16

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#16
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. P48

    Plasmid

    #212155

    Purpose

    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Insert

    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    PgyrA

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYTK076

    Plasmid

    #65183

    Purpose

    Encodes HIS3 as a Type 6 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    HIS3
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK1006 (pSL5)

    Plasmid

    #191001

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    CP25

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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