31,175 results
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Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
ExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutL
Plasmid#86607PurposeE. coli MutL expression plasmidDepositorInsertE. coli MutL
Tagshis-tag and ald-tagExpressionBacterialPromoterT7Available SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(414)/GFP-AUT7(414)
Plasmid#49424PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterATG8Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rac1
Plasmid#55692PurposeExpression of mouse Rac1DepositorAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-Bst-LF-Idaho
Plasmid#170469PurposeExpresses His-tagged Bst-LF-Idaho in E. coliDepositorInsertBst-LF-Idaho
TagsN-terminal His-tagExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCW02
Plasmid#72301PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG28-J23151-AraC
Plasmid#102453PurposeExpresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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dKatushka-pBAD
Plasmid#54775PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635DepositorTypeEmpty backboneTags6xHis and dKatushkaExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_minus_cer-region
Plasmid#175297PurposeProduction of HaloTag fusion proteinDepositorTypeEmpty backboneUseCell free expressionTagsHaloTag-6xHisExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM190
Plasmid#69614Purposeinducible gene expression in StreptomycesDepositorInsertsaphII
tsr
rep
sso
TipA promoter
dso
UseStreptomyces- e. coli shuttle vectorExpressionBacterialAvailable SinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSG320
Plasmid#115597Purposesurface-displayed Nb2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
TagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS
Plasmid#191457PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent proteinDepositorInsertG-Ca-FLITS
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK1037
Plasmid#8833DepositorInsertTVMV protease, catalytic domain
ExpressionBacterialAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET PPL His6 LIC cloning vector (2J-T)
Plasmid#37182DepositorTypeEmpty backboneTagsHis6 and PPLExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only