30,811 results

1. NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

   Plasmid

   #136543

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Tags

   ANXA2 (23-339) and His-tag

   Expression

   Bacterial

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbB7k-GFP

   Plasmid

   #35360

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL4369 (pEffector_TnpB2,ωRNA)

   Plasmid

   #210871

   Purpose

   Expresses GstTnpB2 and native ωRNA from constitutive promoters

   Depositor

   Samuel H. Sternberg

   Article

   Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

   Insert

   GstTnpB2
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGC4593

   Plasmid

   #110206

   Purpose

   Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

   Depositor

   Adam Arkin, Guillaume Cambray

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Inserts

   TEV protease
   aaRS
   

   Expression

   Bacterial

   Promoter

   Pbad and Ptet

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_FYN-1/1

   Plasmid

   #91413

   Purpose

   Protein expression and purification of human SH3 domain construct FYN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   FYN-1/1 (FYN Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPro18

   Plasmid

   #17806

   Depositor

   Jay Keasling

   Article

   Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIntegrase_7

   Plasmid

   #60578

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_7
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET14b_tev_PSG

   Plasmid

   #59731

   Purpose

   Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria

   Depositor

   Alan Fanning

   Article

   Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

   Insert

   TJP1 (TJP1 Human)
   

   Tags

   HIS and TEV

   Expression

   Bacterial

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pTU398

   Plasmid

   #178980

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   gRAMP
   

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPEPY-Plac

    Plasmid

    #122634

    Purpose

    BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. hnRNPA2_RRM2

    Plasmid

    #157866

    Purpose

    express His tagged hnRNPA2 RRM2

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB092a

    Plasmid

    #79217

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TT 3XFLAG-wtGATA6-3XAU1

    Plasmid

    #72612

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Promoter

    TRE tight promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE141

    Plasmid

    #118493

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCarCBADE_ProB(I69E)A

    Plasmid

    #117756

    Purpose

    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    

    Expression

    Bacterial

    Promoter

    PLac(UV5)

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFPV25.1_rpsM_mCerulean3

    Plasmid

    #124904

    Purpose

    rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    rpsM promoter and mCerulean3
    

    Expression

    Bacterial

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLITMUS-rpoN-cI-J23106-geneIII

    Plasmid

    #80843

    Purpose

    Phagemid (encodes M13 gene III and lambda cIopt).

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    Lambda cI
    

    Expression

    Bacterial

    Promoter

    BBa_J23106 and rpoN

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDuex2-iBB-GECO1

    Plasmid

    #194216

    Purpose

    Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

    Depositor

    Robert Campbell

    Article

    Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

    Insert

    iBB-GECO1
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GB1-ALIX(P801G)

    Plasmid

    #180025

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EHMT1 (2IGQ)

    Plasmid

    #25504

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EHMT1 (EHMT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal domain, residues 951-1235

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits