29,903 results

1. pPath-Violacein-pT7native

   Plasmid

   #200002

   Purpose

   Violacein pathway. pT7-native CDS sequence

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Pathways
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAR357_SFP_pCDF-1b

   Plasmid

   #122851

   Purpose

   Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   Sfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXW117Ptet2-gfp

   Plasmid

   #160818

   Purpose

   aTc-responsive single-layered circuit

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

   Insert

   PJ23117-30tetR-t-Ptet2-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23117, Ptet2

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUM-Kan-MCS2-gfp

   Plasmid

   #109290

   Purpose

   Expresses high levels of gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Kanamycin

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AsCpf1-2NLS

   Plasmid

   #102565

   Purpose

   bacterial expression of AsCpf1-2NLS

   Depositor

   Jennifer Doudna

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   AsCpf1
   

   Use

   Tags

   6xHis-MBP-TEV and HA-2xSV40NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pE2n

   Plasmid

   #17456

   Purpose

   modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAT217

   Plasmid

   #134885

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

   Plasmid

   #110560

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. human p53-(1-393)

    Plasmid

    #24859

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Full length.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge