30,223 results

1. pJL1-mTFP1

   Plasmid

   #102635

   Purpose

   In vitro expression of mTFP1 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-p53 R175H

   Plasmid

   #39480

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Insert

   TP53 (TP53 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   P72R and R175H

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pTriEx-mCherry-LOVSD

   Plasmid

   #81033

   Purpose

   Mammalian expression of the super-dark state of LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOVSD
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   C450A, L514K, G528A, L531E, N538E

   Promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPBO.ACT003

   Plasmid

   #182713

   Purpose

   aTc inducible dCasRx-IF3

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 3

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCol1a1-frt (FVB)

   Plasmid

   #63575

   Purpose

   Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

   Depositor

   Ivo Huijbers

   Article

   Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

   Insert

   Col1A-frt-hygro-pA
   

   Use

   Mouse Targeting; Frt/flpe

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   None

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ETS2_pet28a

   Plasmid

   #131664

   Purpose

   Bacterial expression of ETS gene ETS2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETS2 (ETS2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 4Z3C (TET3)

   Plasmid

   #119969

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   TET3 (TET3 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 48-98

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pJKW0770

   Plasmid

   #119023

   Purpose

   E.coli expression plasmid for PmSPS1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmSPS1
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUMO His6 SUMO PYL1 (36-211)

   Plasmid

   #177843

   Purpose

   Bacterial Expression of PYL1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 1 (PYL1 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACYCDUET-StrepSUMO-DaPFOR

    Plasmid

    #217819

    Purpose

    Expresses DaPFOR in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    pACYCDUET-StrepSUMO-DaPFOR
    

    Use

    Tags

    StrepSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p4286

    Plasmid

    #198152

    Purpose

    bacterial expression of sseJ::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDH42(CUG/A)

    Plasmid

    #154132

    Purpose

    Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    engineered bacterial rRNAs operon under control of pL promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKCiR5

    Plasmid

    #149462

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinR(AM)-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCin

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-6xHis-BglII

    Plasmid

    #196654

    Purpose

    Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCoofy31

    Plasmid

    #43993

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6 and OneStrep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGST-RXRalpha

    Plasmid

    #16547

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Insert

    RXR alpha (RXRA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal DNA-binding domain: aa 1-224

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHL 246

    Plasmid

    #53014

    Purpose

    AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    fimS (off orientation)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. 6CDG (GID4)

    Plasmid

    #119961

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 124-289

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pNRB150

    Plasmid

    #36451

    Depositor

    Douglas Bishop

    Article

    Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

    Insert

    DMC1 (DMC1 Budding Yeast)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge