30,223 results

1. pTL2-IncB-GFP11x7

   Plasmid

   #113031

   Purpose

   Expresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoter

   Depositor

   Kevin Hybiske

   Article

   Wang et al Pathog Dis. 2018 Mar 1;76(2). pii: 48301

   Insert

   IncB (incB Chlamydia trachomatis)
   

   Use

   Tags

   GFP11x7 and flag

   Expression

   Bacterial

   Mutation

   Promoter

   tet inducible

   Available since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-51b(+)-Caprola_09

   Plasmid

   #194670

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE-mcs

   Plasmid

   #20320

   Depositor

   Dirk Schnappinger

   Article

   Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

   Insert

   multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL-1

   Plasmid

   #180422

   Purpose

   Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

   Depositor

   Nancy Moran

   Article

   Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Red Chromprotein gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRiboI-Sec-APEX2m

   Plasmid

   #176845

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB23

   Plasmid

   #103058

   Purpose

   medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

   Depositor

   Dirk Bumann

   Article

   Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

   Insert

   GFP-OVA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hydrogen peroxide-inducible promoter katGp

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDE43-MCK

   Plasmid

   #49523

   Purpose

   Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMyCA-mCherry

   Plasmid

   #84272

   Purpose

   mCherry fluorescence protein expression using M. smegmatis

   Depositor

   Young-Hwa Song

   Article

   Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

   Insert

   mCherry
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Acetamidase

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHelper-CBE-sgRNA

   Plasmid

   #221137

   Purpose

   Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with Cas9 handle
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119(SpeI)

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCOLA-Gent-yibDp-Erv1p

    Plasmid

    #202483

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7C-HSPA1L

    Plasmid

    #177660

    Purpose

    Recombinant protein synthesis of human HSPA1L

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA1L (HSPA1L Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQTEV-PSMD10

    Plasmid

    #31332

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PIAS2

    Plasmid

    #40743

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIAS2 (PIAS2 )
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. Lapronic/pRSETB

    Plasmid

    #140755

    Purpose

    FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

    Depositor

    Luis Felipe Barros

    Article

    Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

    Insert

    Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. L1-P941L

    Plasmid

    #15498

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-P941L (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Proline 941 changed to Leucine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pMRE-Tn5-136

    Plasmid

    #118522

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. roGFP_iE_C147S_pQE30

    Plasmid

    #48631

    Purpose

    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    roGFP1
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    insertion E147B, C147S

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIBB-mEGFP-IRES-puro2b

    Plasmid

    #26462

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    pIBB
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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