30,875 results

1. pGEX-2T-Cdc42-T17N

   Plasmid

   #12970

   Depositor

   Gary Bokoch

   Insert

   Cdc42 DN (CDC42 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T17N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pHS0663 pET45b(+) KraIscB-1 TwinStrep

   Plasmid

   #176536

   Purpose

   Bacterial expression of KraIscB-1-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   KraIscB-1
   

   Tags

   TwinStrep

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD564

   Plasmid

   #153026

   Purpose

   dxCas9(3.7), MCP-SoxS(R93A/S101A)

   Depositor

   Jesse Zalatan

   Article

   Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

   Insert

   dxCas9(3.7), MCP-SoxS(R93A/S101A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Available Since

   Aug. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB078d3

   Plasmid

   #79203

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Stat5A-SH2

   Plasmid

   #46515

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat5A (STAT5A Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 582-693

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-mmA1

   Plasmid

   #61090

   Purpose

   expression of fusion protein: MBP-hnRNP A1

   Depositor

   Sean Ryder

   Article

   Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

   Insert

   hnRNP A1 (Hnrnpa1 Mouse)
   

   Tags

   H6_MBP

   Expression

   Bacterial

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCCM

   Plasmid

   #162708

   Purpose

   Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIA033

   Plasmid

   #200420

   Purpose

   thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

   Depositor

   Rut Carballido-López

   Article

   Altinoglu et al bioRxiv 2023

   Type

   Empty backbone

   Tags

   bsPAmCherry

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIA034

   Plasmid

   #200421

   Purpose

   amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.

   Depositor

   Rut Carballido-López

   Article

   Altinoglu et al bioRxiv 2023

   Type

   Empty backbone

   Tags

   bsdronPA

   Expression

   Bacterial

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB2-mRuby3 Addgene

    Plasmid

    #104005

    Purpose

    Plasmid encoding mRuby3 Addgene

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mRuby3 Addgene
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pet28a-ClbB-NRPS-NHis

    Plasmid

    #49217

    Purpose

    NRPS module of ClbB containing CAT domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbB-NRPS
    

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa4-1080 of reference sequence NP_754362…

    Promoter

    T7

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4QQD

    Plasmid

    #61947

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1:SDC130-H02:C39674 (UHRF1 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 884 pGEX2T PTEN G129E

    Plasmid

    #10747

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G129E

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-PGC1(1-400)

    Plasmid

    #1029

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains only aa1-400

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

15. TTR C10S S85C

    Plasmid

    #184410

    Purpose

    bacterial expression of TTR

    Depositor

    Peter Wright

    Article

    Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZFYVE16

    Plasmid

    #39099

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ZFYVE16 (ZFYVE16 Human)
    

    Tags

    His6-Zb-TEV

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pJS102

    Plasmid

    #118281

    Purpose

    Expresses GFPmut2 with low cell-to-cell variation and lactose induction

    Depositor

    Zach Hensel

    Article

    Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Promoter

    pLlacO-1

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Stat3-SH2

    Plasmid

    #46513

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat3 (STAT3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 584-700

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIA1400

    Plasmid

    #172519

    Purpose

    Expression of SARS-COV-2 Nsp12 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-hygMV

    Plasmid

    #73891

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    hygMV
    

    Expression

    Bacterial and Yeast

    Promoter

    pombe TEF

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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