31,184 results

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pBAD/HisD-LSSmKate1

Plasmid

#31908

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate1

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his

Plasmid

#210405

Purpose

For expression of VHH with C-terminal his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pE4n

Plasmid

#17458

Purpose

modified pENTR vector for N-terminal GST tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

GST and Thrombin site

Expression

Bacterial, Insect, Mammalia…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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HC25 pComb3xss anti-Homer1 nanobody

Plasmid

#134718

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDB038a

Plasmid

#99211

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT

Expression

Bacterial

Mutation

amber codons at positions 29 and 184 of gIII

Promoter

Ppsp and PproK

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-Katushka

Plasmid

#104008

Purpose

Plasmid encoding Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_GATE-16

Plasmid

#73518

Purpose

For recombinant expression of human GATE-16/GABARAPL2 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GATE-16 (GABARAPL2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pZ8-Prp

Plasmid

#74065

Purpose

pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

Propionate inducible promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

prp

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pXD69m1-TetW

Plasmid

#191246

Purpose

E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET-45b-HP1a-mCherry

Plasmid

#185012

Purpose

Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

CBX5 (HP1a) (Cbx5 Mouse)

Expression

Bacterial

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Tags

His6tev

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pST39-Ubc13-MMS2

Plasmid

#184807

Purpose

Bacterial expression of yeast UEV-E2 complex

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Ubc13, MMS2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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A3RXB7

Plasmid

#177694

Purpose

expresses a N-acetyl hexosamine oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

N-acetylhexosamine oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAcCAST-sgRNA_entry (CJT83)

Plasmid

#181785

Purpose

Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pKRR5

Plasmid

#149468

Purpose

Broad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (2R-T)

Plasmid

#29717

Depositor

Scott Gradia

Type

Empty backbone

Tags

StrepII-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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