33,128 results
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Plasmid#238209PurposeExpresses native FpTasA TIGR locus. CmRDepositorInsertFpTasA
ExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK004
Plasmid#233641PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagA
Tags6-HisExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
NP-11-64-1
Plasmid#234359PurposeExpressing rubisco and prk in ΔrpiDepositorInsertR.rubrum rubisco
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_N-myc
Plasmid#234045PurposeBacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage siteDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_MAX
Plasmid#234044PurposeBacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage siteDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRExpressionBacterialPromoterPgdh and PxylAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCV019-catP
Plasmid#232825PurposeRandom transposon mutagenesis vector for Bacteroidales species - chloramphenicolDepositorInsertcatP
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
KPC2-pNic-Bsa4
Plasmid#231082Purposeexpresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tagDepositorAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZP06
Plasmid#232320PurposepZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.DepositorInsertPfdx-E
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-227_Cacnes_mCerulean
Plasmid#225614PurposeReplicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.DepositorInsertmCerulean
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCN38-kimA-yfp
Plasmid#227635Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF2
Plasmid#228776PurposeProtein expression plasmid for YTH domain of YTHDF2 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-M1-di-Ub(G76V)-HOIL-1
Plasmid#229539PurposeConstitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coliDepositorTags6xHis-3CExpressionBacterialMutationChanged Gly 76 to Val and changed Gly 76 to ValPromoterT7Available SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
TagsHA tag and Histidine tagExpressionBacterialPromoterT7Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only