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Bacterial Expression

30,235 results

Showing: 8381 - 8400 of 30235 results

  3. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  6. pET28a-TGFα-GSY-SPM

    Plasmid
    #163615
    Purpose
    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.
    Depositor
    Mark Howarth
    Article
    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:
    Insert
    TGFa-GSY-SPM (TGFA Synthetic, Human)
    Use
    Tags
    C-tag and His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    163615-plasmid-map-sequence-id-323634

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  7. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  9. proinsulin-moxGFP2

    Plasmid
    #218957
    Purpose
    Expresses proinsulin fused to moxGFP2 in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-moxGFP2
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218957-plasmid-map-sequence-id-446163

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  10. proinsulin-mGold

    Plasmid
    #218953
    Purpose
    Expresses proinsulin fused to mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-mGold
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218953-plasmid-map-sequence-id-446158

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  12. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Use
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  15. pET11b-MIF Y99F

    Plasmid
    #149439
    Purpose
    Expression of MIF Y99F in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99F
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149439-plasmid-map-sequence-id-311578

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  19. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid
    #100018
    Purpose
    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    cpsfGFP-LSSmOrange
    Use
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Leu and Glu N-term linker and Leu and Pro C-term …
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100018-plasmid-map-sequence-id-194292

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  20. p35u4

    Plasmid
    #174786
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system
    Depositor
    Katherine Berry
    Article
    Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126
    Insert
    MS2 coat protein
    Use
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174786-plasmid-map-sequence-id-361842

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Showing: 8381 - 8400 of 30235 results