33,526 results

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Publication

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (DNM1L Human)

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINO_C1

Plasmid

#171924

Purpose

Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Publication

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_C1

Tags

His6

Expression

Bacterial

Available since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PdnaK GFP pZa

Plasmid

#170073

Purpose

GFP expression under the control of E. coli dnaK promoter

Depositor

Urartu Seker

Publication

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKD280.7

Plasmid

#173172

Purpose

Expresses SO_4387

Depositor

Jeffrey Tabor

Publication

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

SO_4387

Expression

Bacterial

Promoter

J23107

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-tns

Plasmid

#170628

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Publication

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTnsABC

Expression

Bacterial

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41-4GS-LaG-2

Plasmid

#172766

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSunS

Plasmid

#171672

Purpose

SunS expression in E. coli

Depositor

Wilfred van der Donk

Publication

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA_crE6G2

Plasmid

#172929

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

Depositor

Arnold Boersma

Publication

Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

Insert

CrowdingSensorE6G2

Tags

His

Expression

Bacterial

Mutation

Val317Gly, Met316Gly

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-mVenus-I

Plasmid

#163763

Purpose

Expresses Streptomyces codon-optimised mVenus-I

Depositor

Paul Freemont, Simon Moore

Publication

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mVenus-I

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

SP44

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTU1-B-SP44s-GUS

Plasmid

#172430

Purpose

Expresses Streptomyces codon-optimised beta-D-glucuronidase

Depositor

Paul Freemont, Simon Moore

Publication

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

beta-D-glucuronidase

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

SP44s

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT207

Plasmid

#134884

Purpose

Bacterial expression

Depositor

Thomas Howard

Publication

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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