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32,457 results

Showing: 8721 - 8740 of 32457 results

  1. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  4. pBT3000

    Plasmid
    #122536
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122536-plasmid-map-sequence-id-238187

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  5. UBC32

    Plasmid
    #25213
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc32.001.158 (UBE2K Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25213-plasmid-map-sequence-id-461309

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  7. SOCS2A-c016

    Plasmid
    #53689
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    SOCS2A
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid
    #101217
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101217-plasmid-map-sequence-id-197215

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  9. Rec-PhoA/CsgA

    Plasmid
    #170787
    Purpose
    Recombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinase
    Depositor
    Urartu Seker
    Article
    Ahan et al Chembiochem. 2019 Jul 15;20(14):1799-180
    Inserts
    csgA (csgA )
    phoA (phoA )
    Use
    Synthetic Biology
    Tags
    HHHHHH
    Expression
    Bacterial
    Promoter
    Inverted proD with Bxb1recognition sites and Inve…
    Available Since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170787-plasmid-map-sequence-id-343131

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  14. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

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  15. VRK1

    Plasmid
    #39067
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    VRK1 (VRK1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation K34A, K35A, E3…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pTEV5_ RpMatB_1

    Plasmid
    #78205
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7
    Insert
    matB (His6 C106A R286C Q457C K488A)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A R286C Q457C K488A
    Promoter
    T7
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. CAMK2D

    Plasmid
    #38987
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2D (CAMK2D Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pJJS005

    Plasmid
    #188328
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::mNeonGreen::Linker
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188328-plasmid-map-sequence-id-370751

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  20. pNR64

    Plasmid
    #79540
    Purpose
    Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79540-plasmid-map-sequence-id-148741

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Showing: 8721 - 8740 of 32457 results