30,880 results

1. pT7C-DNAJB6

   Plasmid

   #177657

   Purpose

   Recombinant protein synthesis of human DNAJB6

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   DNAJB6 (DNAJB6 Human)
   

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

   Plasmid

   #181788

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX ShcD-SH2

   Plasmid

   #46488

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   ShcD (SHC4 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 512-623

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. NR4A1

   Plasmid

   #25477

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NR4A1 (NR4A1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. csr-1_son-1_mTagBFP2

   Plasmid

   #191746

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and mTagBFP2

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAN4023

   Plasmid

   #74701

   Purpose

   Output plasmid: PBM3R1-YFP

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PBM3R1-YFP
   

   Expression

   Bacterial

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SENP5 (catalytic domain)

   Plasmid

   #16358

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP5 catalytic domain (SENP5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 567 to 755 catalytic domain of human S…

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pJMP2822

   Plasmid

   #160670

   Purpose

   Mobile CRISPRi sfGFP 'test', empty sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   empty sgRNA (BsaI)
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. human p53-(1-393)-N268D

   Plasmid

   #24861

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Asparagine 268 mutated to Aspartate

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pRSETb-tVYN-TmΔ

    Plasmid

    #149694

    Purpose

    High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-TmYcgRΔ-NLucΔ4
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCOM4

    Plasmid

    #49798

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Tags

    S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels

    Plasmid

    #204801

    Purpose

    Bacterial expresison of Enterovirus D68 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    D68 2A protease
    

    Expression

    Bacterial

    Available Since

    Dec. 11, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pYZ_3WJrep_N24_switch

    Plasmid

    #132725

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N24_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. UBC13-PGEX4T

    Plasmid

    #25464

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    Ubc13 (UBC13, MMS2 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET16b-Mms2

    Plasmid

    #18893

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
    

    Tags

    HIS10

    Expression

    Bacterial

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

19. GB1-His-TET1-848-952(pL91)

    Plasmid

    #124388

    Purpose

    E.Coli produced TET1-848-952 purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-848-952 wildtype (TET1 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDuet-T7-Ct-WspR

    Plasmid

    #79166

    Purpose

    Dual expression of Ct biosensor and WspR WT under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    WspR (wspR P. areuginosa)
    T7-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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