30,249 results

1. pBbS7k-RFP

   Plasmid

   #35321

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-mVenus-Zdk1-RhoA Q63L

   Plasmid

   #81046

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-mCherry-LOV2 V416I

   Plasmid

   #81045

   Purpose

   mammalian expression of LOV V416I

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV V416I
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   V416I

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet-1 TIM8,13

   Plasmid

   #170282

   Purpose

   Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

   Insert

   yeast Tim8 and Tim13 (TIM8 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag on Tim13 (on bac…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJS003

   Plasmid

   #188326

   Purpose

   PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::mCherry::Linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIA1364

   Plasmid

   #172401

   Purpose

   Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp9 (ORF1ab Synthetic)
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Clover-pBAD

   Plasmid

   #54575

   Purpose

   Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   6xHis and Clover

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pCCL-ReDNAP (Y427A)

   Plasmid

   #52346

   Purpose

   CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   Recoded TP-DNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   REV1

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

   Plasmid

   #107582

   Purpose

   B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   GFP+
   

   Use

   Synthetic Biology; E. coli and bacillus shuttle v…

   Tags

   B. megaterium DSM319 xylA leader sequence (MTSSKI…

   Expression

   Bacterial

   Mutation

   F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

   Promoter

   fba promoter Bacillus megaterium DSM319

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKM631

    Plasmid

    #17727

    Depositor

    David Waugh

    Article

    Kapust et al Biochem Biophys Res Commun. 2002 Jun 28.

    Insert

    NusG (nusG Aquifex aeolicus)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MP-P6

    Plasmid

    #69665

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR dinB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p2H12Matry-blind

    Plasmid

    #221181

    Purpose

    cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen-Matry(blind)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIC-NHSTIIT-AVITAG-GG

    Plasmid

    #215802

    Purpose

    Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pHIT179

    Plasmid

    #204510

    Purpose

    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

    Depositor

    Yuji Kohara

    Article

    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

    Insert

    csr-1a (C. elegans) (csr-1 Nematode)
    

    Use

    Tags

    6xHis, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BASIC_SEVA_66.11

    Plasmid

    #194213

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pHH0103_NR4A3_Hormone-recep

    Plasmid

    #109952

    Purpose

    Protein expression and purification of NR4A3_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR4A3_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_RORB_Hormone-recep

    Plasmid

    #109958

    Purpose

    Protein expression and purification of RORB_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RORB_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_HNF4G_Hormone-recep

    Plasmid

    #109916

    Purpose

    Protein expression and purification of HNF4G_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    HNF4G_Hormone-recep (HNF4G Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC18T-mini-Tn7T-Zeo-ecfp

    Plasmid

    #65038

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge