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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEB2-mScarlet

Plasmid

#104006

Purpose

Plasmid encoding mScarlet

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Publication

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Publication

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Tol2-mpeg1-dendra2

Plasmid

#51462

Purpose

expresses photoconvertible dendra in macrophages

Depositor

Anna Huttenlocher

Publication

Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

Insert

mpeg-dendra

Expression

Bacterial

Promoter

mpeg1

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Publication

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAD48-daf-2 RNAi

Plasmid

#34834

Depositor

Cynthia Kenyon

Publication

Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Insert

daf-2 (daf-2 Nematode)

Use

RNAi

Expression

Bacterial

Available since

June 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
GST-PRMT7

Plasmid

#34693

Depositor

Steven Clarke

Publication

Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pZS*12-MS2-GFP

Plasmid

#26245

Depositor

Philippe Cluzel

Publication

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

MS2-GFP

Expression

Bacterial

Mutation

five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

Available since

Nov. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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WGA-D

Plasmid

#17989

Depositor

Natasha Raikhel

Publication

Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

Insert

wheat germ agglutinin isolectin D

Expression

Bacterial

Mutation

N/A

Available since

July 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDEST17-UbE2A

Plasmid

#15780

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2A (UBE2A Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Publication

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Publication

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQLinkHD

Plasmid

#13668

Depositor

Konrad Buessow

Publication

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

Gateway cassette

Use

Co-expression

Tags

His

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GEV 2

Plasmid

#12616

Depositor

John Louis

Publication

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-RIP

Plasmid

#11972

Depositor

Jon Ashwell

Publication

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

RIP (Ripk1 Mouse)

Tags

GST

Expression

Bacterial

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Publication

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
Human LFA-1 alpha

Plasmid

#8630

Depositor

Timothy Springer

Publication

Larson et al J Cell Biol 1989 Feb;108(2):703-12.

Insert

LFA1 alpha (ITGAL Human)

Expression

Bacterial

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Publication

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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