33,526 results
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Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneTagsGST and HAExpressionBacterialPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
ExpressionBacterialPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTHSSe_51
Plasmid#109249Purposep15A BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG042
Plasmid#113998PurposeVector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectorsDepositorInsertTetracycline
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOGG014
Plasmid#113988PurposeVector construction module. Endlinker/terminator module to circularize plasmid following Part 4DepositorInsertEndlinker/terminator Part 4
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
B5-2
Plasmid#111481PurposeArg-tRNA synthetaseDepositorInsertArg-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
6CC8 (MBD3)
Plasmid#119959PurposeBacterial expression for structure determinationDepositorInsertMBD3 (MBD3 Human)
TagsGSTtag, 6xHis and ThrombinExpressionBacterialMutationMBD domain (contains residues 1-71)PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1335
Plasmid#119269Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1185
Plasmid#119255Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11d_Rev
Plasmid#119322PurposeExpression of protein Rev from HIVDepositorInsertRev (rev HIV B1)
ExpressionBacterialAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1161
Plasmid#119251Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfCFPBb
Plasmid#118232PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFPDepositorInsertflagellin promoter driven msfCFP
ExpressionBacterialAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
ExpressionBacterialPromoterPpro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB088h
Plasmid#79215PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB088c
Plasmid#79213PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)
Plasmid#114077PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R/S542R/K607RAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)
Plasmid#114075PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548V and N552RAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by