32,684 results
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Plasmid#198309PurposeNegative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)DepositorInsertPVCpnfĪpvc15_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCri_L706-antiSH2-OptoMB
Plasmid#159935PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
ExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDE43-MCK
Plasmid#49523PurposeKanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
STK25
Plasmid#39075PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah3
Plasmid#122570PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-II
Plasmid#17633DepositorExpressionBacterialAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_2
Plasmid#118226PurposeEmpty E. coli - B. burgdorferi shuttle vector; kanamycin resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB038a
Plasmid#99211PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
ExpressionBacterialMutationamber codons at positions 29 and 184 of gIIIPromoterPpsp and PproKAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
TagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-msYFP
Plasmid#218960PurposeExpresses Hisx6-Tagged msYFP in bacteriaDepositorInsertmsYFP
Tags6X-HisExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM263-USP7
Plasmid#196365Purposeprotein expression of the GST-tagged catalytic domainDepositorInsertUSP7 catalytic domain
TagsHis6-GST-TEVExpressionBacterialMutationcontains only S207-E560PromoterT7Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
P48
Plasmid#212155PurposePlasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.DepositorInsertsuperfolder green fluorescent protein, secondary alcohol dehydrogenase
Tags6x HisExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP4F2 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/spoR
Plasmid#73631PurposeProduces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tagDepositorInsertguanylate kinase
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAT1A
Plasmid#38852PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HC25 pComb3xss anti-Homer1 nanobody
Plasmid#134718PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
TagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alaniā¦PromoterT7Available SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFL1 (CFL1A-c002)
Plasmid#98237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCFL1A (CFL1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00241:Cofilin_ADF 27-148PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only