30,249 results

1. pENTR2B-dCas9-KRAB

   Plasmid

   #115547

   Purpose

   Entry cassette for dCas9-KRAB

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Insert

   dCas9-KRAB
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEB1-mEYFP

   Plasmid

   #103985

   Purpose

   Plasmid encoding mEYFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mEYFP
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S65G, V68L, …

   Promoter

   proC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMTTH+mEGFP-LANA

   Plasmid

   #157801

   Purpose

   Expression of mEGFP and LANA peptide fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-LANA
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP26-1A(sfGFP)

   Plasmid

   #126973

   Purpose

   Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET11a-STEPtag

   Plasmid

   #159373

   Purpose

   Expresses STEPtag in E. coli

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMrfp

   Plasmid

   #59773

   Purpose

   Derived from pCM62; ampR, tetR, PBAD - rfp

   Depositor

   Nathan Hillson

   Article

   Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

   Insert

   rfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BB1_12_YlpGPD

   Plasmid

   #117820

   Purpose

   Promoter pGPD of Y. lipolytica for GoldenMOCS

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Insert

   YlpGPD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBBR4pemIK

   Plasmid

   #126928

   Purpose

   Broad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selection

   Depositor

   Lindsey Burbank

   Article

   Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAJM.721

    Plasmid

    #108522

    Purpose

    PSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PSalTTC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTriEX-Antenna-GDI Cdc42

    Plasmid

    #101681

    Purpose

    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    Cdc42
    

    Use

    Tags

    6xHis and FRET Antenna (mCerulean-cpVenus)

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BAX KO vector

    Plasmid

    #16575

    Depositor

    Bert Vogelstein

    Article

    Zhang et al Science. 2000 Nov 3. 290(5493):989-92.

    Insert

    BAX knockout (BAX Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

14. RP1B-PP1gamma

    Plasmid

    #51770

    Purpose

    Expresses PP1 gamma residues 7-323

    Depositor

    Wolfgang Peti

    Insert

    pp1 gamma
    

    Use

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 gamma residues 7-323

    Promoter

    T7-lac

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSB1K3_pBen-sfGFP_J23101-mRFP

    Plasmid

    #128126

    Purpose

    Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBen (benzoate responsive promoter)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

    Plasmid

    #191647

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TOPO CFP

    Plasmid

    #68489

    Purpose

    TOPO construct expressing CFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cyan Fluorescent Protein
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJexpress414_OmpA171_WT

    Plasmid

    #62646

    Purpose

    Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop

    Depositor

    Timothy Whitehead

    Article

    Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

    Insert

    OmpA
    

    Use

    Synthetic Biology

    Tags

    6His and FlAsH

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET-NtLAtC60αβC20

    Plasmid

    #160873

    Purpose

    Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: pBAD, chaperones: T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PTPN11

    Plasmid

    #38965

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only