30,249 results

1. pGEX4T3-GST-PolB(WT)

   Plasmid

   #177131

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJL2

   Plasmid

   #199377

   Purpose

   Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   GCaMP6f::SL2::mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no mutations

   Promoter

   Available since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMJ839

   Plasmid

   #39317

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (NMA0631 Neisseria meningitidis)
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pAN-PBAD-sgRNA-A2NT

   Plasmid

   #62257

   Purpose

   expression of A2NT sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   A2NT sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-29

   Plasmid

   #172753

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-29
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK509

   Plasmid

   #110610

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pH6HTC_hsHUR-Halo

   Plasmid

   #175303

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HUR (ELAVL1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-PEPD

   Plasmid

   #31328

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   peptidase D (PEPD Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. CBP TAZ1(340-439)

   Plasmid

   #173760

   Purpose

   bacterial expression of mouse CBP TAZ1 domain

   Depositor

   Peter Wright

   Article

   De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

   Insert

   CBP TAZ1 (Crebbp Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Use

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Spun Hsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD3-proD/mClover/nat

    Plasmid

    #120330

    Purpose

    Bacterial mClover labeling

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mClover
    

    Use

    Tags

    D103N (see comment below)

    Expression

    Bacterial

    Mutation

    Promoter

    (None)

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC-MBP-TEV-GG

    Plasmid

    #215794

    Purpose

    Cloning of target gene with N terminal MBP tag and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHflu2

    Plasmid

    #203880

    Purpose

    pHI1 with insertion of ampicillin-resistance gene

    Depositor

    Bogumil Karas

    Insert

    broad-spectrum class A beta-lactamase TEM-116
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB37

    Plasmid

    #202602

    Purpose

    Arabinose inducible expression vector expressing retron Rcat-Sen2

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Insert

    RcaT-Sen2 toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. As2

    Plasmid

    #123160

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS84, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pES133

    Plasmid

    #127552

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Insert

    F5-flanked tetracycline resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Multiple SNPs in tetA (originally from pBR322) to…

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. RHOB

    Plasmid

    #38801

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RHOB (Rhob Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCD227

    Plasmid

    #113318

    Purpose

    AraC-pBAD-dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    R93A

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD_His B_QuasAr1

    Plasmid

    #64135

    Purpose

    Fluorescent reporter for membrane voltage with improved brightness

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZIKV_Sensor_32B_LacZ

    Plasmid

    #75007

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_32B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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