33,128 results
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Plasmid#55765PurposeEncoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cellsDepositorInsertY-GECO1.0
UseLab constructedTags6x His tagExpressionBacterialPromoterpBADAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS4
Plasmid#55751PurposeProduces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 1-59 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hTDG-K330A
Plasmid#52282PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GSTDepositorInserthTDG (TDG Human)
TagsGyrA-intein-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
msEGFP-PACR_pRSETB
Plasmid#55775PurposeGenetically encoded caged Ca2+DepositorInsertPACR
Tags6xHis and msEGFPExpressionBacterialPromoterT7 promoterAvailable SinceJuly 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pET His6 GSSGSS NusA TEV LIC cloning vector (2NTL)
Plasmid#55214DepositorTypeEmpty backboneTags6xHis, GSSGSS linker, NusA, and TEV cleavage site…ExpressionBacterialPromoterT7Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hchk2 (kin-)-PUNI10
Plasmid#41908DepositorAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK1 C121S
Plasmid#53164PurposeExpresses GST-HA tagged human MEK1 C121S from bacterial expression vectorDepositorAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mOrange pHis-//
Plasmid#53304PurposeEmpty vector to express N-terminal His-mOrange-tagged fusion protein.DepositorTypeEmpty backboneTagsHis and mOrangeExpressionBacterialAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-IV
Plasmid#53440PurposeDerivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IVDepositorInsertagrBD locus (with D29Y mutation in agrD)
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationD to Y mutation of residue 29 of the agrD gene (c…PromoterT7-lacAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
NEK1A-c011
Plasmid#53661Purposebacterial expression plasmidDepositorInsertNEK1A
TagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
MYO5AA-c010
Plasmid#53632Purposebacterial expression plasmidDepositorInsertMYO5AA
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B CA
Plasmid#49528Purposecloning vector for constitutively active chick BMP receptor 1BDepositorInsertBMPR-1B CA (BMPR1B Chicken)
ExpressionBacterialMutationconstitutively active mutation (Q203D); also P2A,…PromoterT3Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialPromoterfimSAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-GG-PUF
Plasmid#52137PurposeReceiving vector for PUM-HD assembly and expression in E.coliDepositorTypeEmpty backboneTagsPUM-HD flanking region 1' and PUM-HD flankin…ExpressionBacterialPromoterT7Available SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
TagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable SinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHnCB
Plasmid#52064Purposeexpresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B
ExpressionBacterialAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
cpc-PHLS (g)
Plasmid#52311PurposeReplaces the cpc operon in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialPromoterSynechocystis endogenous cpc-operon promoterAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only