30,880 results

1. pWzyE-distrepII

   Plasmid

   #196667

   Purpose

   Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Tags

   TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. UMG-SP-1_pET29b

   Plasmid

   #215433

   Purpose

   Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-1
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. UMG-SP-2_pET29b

   Plasmid

   #215434

   Purpose

   Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-2
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCSG28-SH3-588

   Plasmid

   #185920

   Purpose

   Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

   Depositor

   Brian Kuhlman

   Article

   Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

   Insert

   SH3-588
   

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Designed enzyme with novel interface between huma…

   Promoter

   T7

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mUAVbla:tetR 5′

   Plasmid

   #149379

   Purpose

   mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Insert

   tetA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKM2 (3GQY)

   Plasmid

   #25360

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PKM2_1 (PKM Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 19, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. MAP1LC3A

   Plasmid

   #25185

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubh67.001.121 (MAP1LC3A Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pGEX2TK-Osh2-PH

   Plasmid

   #71370

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh2-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 277-398

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pJexpress414_OmpA171_WT

   Plasmid

   #62646

   Purpose

   Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop

   Depositor

   Timothy Whitehead

   Article

   Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

   Insert

   OmpA
   

   Use

   Synthetic Biology

   Tags

   6His and FlAsH

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET22b-EC12WTC9A

    Plasmid

    #47924

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1-4A

    Plasmid

    #13268

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIntegrase_13

    Plasmid

    #60584

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_13
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2-Flag-TTGZFP-FokI-DD

    Plasmid

    #18755

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. RP1B-PP1gamma

    Plasmid

    #51770

    Purpose

    Expresses PP1 gamma residues 7-323

    Depositor

    Wolfgang Peti

    Insert

    pp1 gamma
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 gamma residues 7-323

    Promoter

    T7-lac

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET-3a_mb2m

    Plasmid

    #211368

    Purpose

    Expresses murine b2m

    Depositor

    Adnane Achour

    Insert

    b2m (B2m Mouse)
    

    Expression

    Bacterial

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCasTns(Vch)-entry(BsaI)

    Plasmid

    #190273

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
    

    Expression

    Bacterial

    Mutation

    no

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTG131

    Plasmid

    #198640

    Purpose

    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    Cas9
    

    Expression

    Bacterial

    Promoter

    pPhlF

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-Ava3855

    Plasmid

    #45743

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3855 (Ava_3855 Anabaena variabilis)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pH6HTC_mmHUR-Halo

    Plasmid

    #175311

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HUR (Elavl1 Mouse)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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