32,457 results
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Plasmid#115602Purposesurface-displayed Nb1; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC02
Plasmid#233644PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagB.DepositorInsertsfGFP with c-terminal VitelloTagB
Tags6-HisExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAH107
Plasmid#248727PurposePBT2364 biosensor circuit in pNBU2 integrative Bacteroides plasmidDepositorInsertPBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT2364-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
ExpressionBacterialAvailable SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GCaMP6f
Plasmid#67564Purposebacterial expression of a fluorescent calcium reporterDepositorInsertGCaMP6f
TagsHIS-tagExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045)
Plasmid#235005PurposeBacterial expression of N-terminally 6His tagged E3 ubiquitin-protein ligase (Topors) LCDDepositorInsertE3 ubiquitin-protein ligase (Topors) (TOPORS Human)
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDSG375
Plasmid#115603Purposesurface-displayed Nb2; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1 sortase
Plasmid#106288PurposeExpresses sortase in Ecoli off a T7 promoterDepositorInsertsortase A pentamutant
ExpressionBacterialPromoterT7Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-LY-P2A
Plasmid#214895PurposeExpresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sitesDepositorInsertSPLICSFAST-LDh-LY-P2A
TagsnoneExpressionBacterial and MammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG398
Plasmid#115604Purposesurface-displayed Nb3; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialMutationSee depositor comments belowAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG312
Plasmid#115613Purposesurface-displayed Nb2, Arabinose-inducible; medium-copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mNeptune2.5
Plasmid#104013PurposePlasmid encoding mNeptune2.5DepositorInsertmNeptune2.5
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (MSKGEE LIKENM… M11…PromoterproCAvailable SinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSG290
Plasmid#115609Purposesurface-displayed Nb3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG288
Plasmid#115607Purposesurface-displayed Ag3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
ExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTNT-pT7-tetR-mCherry
Plasmid#140870PurposeT7 promoter driven synthesis of TetR-mCherry fusion proteinDepositorInsertTetR-mCherry expressed with T7 promoter
UseSynthetic BiologyTagsmCherryExpressionBacterialPromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT K842M
Plasmid#154272PurposeBacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationLysine 842 changed to Methionine- bacterial codon…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only