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pJMP2

Plasmid

#79874

Purpose

Bacillus subtilis sgRNA expression vector; integrates into amyE

Depositor

Carol Gross, Jason Peters, Stanley Qi

Publication

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAB1

Plasmid

#62547

Purpose

broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

Depositor

Michael Franklin

Publication

Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

eGFP

Use

Broad host range plasmid

Expression

Bacterial

Promoter

pTrc

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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mKate1.31-pBAD

Plasmid

#54827

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.

Depositor

Michael Davidson

Publication

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Type

Empty backbone

Tags

6xHis and mKate1.31 (aka mKate2)

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Publication

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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USP2

Plasmid

#36894

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP2 (USP2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Available since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles
pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GST-YAP2

Plasmid

#24637

Depositor

Kunliang Guan

Insert

Yap2 (YAP1 Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmr101A-hVPS26

Plasmid

#17636

Depositor

James Hurley

Publication

Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

Insert

VPS26 (VPS26A Human)

Tags

His

Expression

Bacterial

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET15b-Casp8 delta DED

Plasmid

#11827

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 8 minus Death Effecter Domain (CASP8 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Minus Death Defecter Domain (aa 1-216)

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-HIV RT-6xHis-RBS-prot

Plasmid

#159149

Purpose

expresses His-tagged HIV reverse transcriptase and untagged HIV protease

Depositor

Andrea Pauli

Publication

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Inserts

Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease

Tags

His

Expression

Bacterial

Mutation

HIV-1 group M/subtype B – BH10 strain

Available since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Expression

Bacterial

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-TyrRS-His

Plasmid

#124121

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tyrosyl-tRNA ligase (tyrS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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