30,735 results

1. RGS2

   Plasmid

   #38812

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RGS2 (Rgs2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pBAD42-BtuCEDFB

   Plasmid

   #237541

   Purpose

   plasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteins

   Depositor

   Squire Booker

   Article

   Lanz et al Biochemistry. 2018 Mar 6;57(9):1475-1490

   Inserts

   btuB
   btuF
   btuCED
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Sept. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-PmPPA-ET64

   Plasmid

   #234667

   Purpose

   Expression an ELP-tagged PmPPA enzyme

   Depositor

   Xianwei Liu

   Article

   Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

   Insert

   pyrophosphatase
   

   Tags

   ELP

   Expression

   Bacterial

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 10xHis-3C-BRD4(42-459) BD1-BD2

   Plasmid

   #233886

   Purpose

   pRSF-DUET1 for protein overexpression of human BRD4

   Depositor

   Alessio Ciulli

   Article

   Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   10x his 3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF.111

   Plasmid

   #227187

   Purpose

   pORTMAGE-Ec1 + supplement E. coli SSB

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   CspRecT, mutL_E32K, E. coli SSB
   

   Expression

   Bacterial

   Mutation

   mutL E32K

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDC.109

   Plasmid

   #227189

   Purpose

   T7 IPTG-driven Cas9

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   SpyCas9
   

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRoRL

   Plasmid

   #213132

   Purpose

   Riboptoregulator circuit RoRL for blue-light-activated gene expression

   Depositor

   Andreas Moeglich

   Article

   Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB602-TP-mCherry-UnaG

   Plasmid

   #203483

   Purpose

   For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   Plastid transit peptide fused mCherry-UnaG
   

   Expression

   Bacterial and Plant

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b MOSPD2 [282-490]

   Plasmid

   #186469

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [282-490]

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFPC1-EPSIN 1

    Plasmid

    #22228

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    please see depositor comment below

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpyTag003-MBP

    Plasmid

    #133450

    Purpose

    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FlipSuc-90u delta1

    Plasmid

    #14475

    Depositor

    Wolf Frommer

    Article

    Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

    Insert

    FlipThuE W283A
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    N192D, W283A, 13 amino acids deleted from linkers

    Promoter

    T7

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6xHis-SNAP-PI(3,5)P2 Probe

    Plasmid

    #211512

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SnxA-2xPX
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOSIP-KO (KanR, 186)

    Plasmid

    #45985

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWUR 1+2 tetO mut89

    Plasmid

    #80102

    Purpose

    Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

    Depositor

    George Church

    Article

    Shipman et al Science. 2016 Jun 9. pii: aaf1175.

    Inserts

    Cas1 (cas1 )
    Cas2 (cas2 )
    Cas1 (cas1 )
    Cas2 (cas2 )
    

    Expression

    Bacterial

    Mutation

    Q24H, P202Q, G241D, E276D, L297Q

    Available Since

    Dec. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLHCX-gpASNase1

    Plasmid

    #121526

    Purpose

    Expresses gpASNase1 in mammalian cells.

    Depositor

    Matthew Vander Heiden, Lucas Sullivan

    Article

    Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

    Insert

    gpASNase1
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJeM1

    Plasmid

    #135088

    Purpose

    rhamnose-inducible expression vector for replication in E. coli and P. putida

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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