30,810 results

1. pGEX CrkL-SH2

   Plasmid

   #46419

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Crkl (CRKL Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 9-108

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2H12Matry-blind

   Plasmid

   #221181

   Purpose

   cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen-Matry(blind)
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-NHSTIIT-AVITAG-GG

   Plasmid

   #215802

   Purpose

   Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pHIT179

   Plasmid

   #204510

   Purpose

   Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

   Insert

   csr-1a (C. elegans) (csr-1 Nematode)
   

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BASIC_SEVA_66.11

   Plasmid

   #194213

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pHH0103_NR4A3_Hormone-recep

   Plasmid

   #109952

   Purpose

   Protein expression and purification of NR4A3_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   NR4A3_Hormone-recep
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_RORB_Hormone-recep

   Plasmid

   #109958

   Purpose

   Protein expression and purification of RORB_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RORB_Hormone-recep
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_HNF4G_Hormone-recep

   Plasmid

   #109916

   Purpose

   Protein expression and purification of HNF4G_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   HNF4G_Hormone-recep (HNF4G Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PTPRE

   Plasmid

   #38950

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPRE (PTPRE Human)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pMTTH+eGFP-bromo5

    Plasmid

    #157804

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-mAIM2-His6

    Plasmid

    #51536

    Purpose

    Express His6-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Tags

    His6 and T7

    Expression

    Bacterial

    Promoter

    lac operator

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid

    #160881

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Expression

    Bacterial

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1-E309K

    Plasmid

    #15487

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-E309K (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 309 changed to Lysine

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pJB37

    Plasmid

    #202602

    Purpose

    Arabinose inducible expression vector expressing retron Rcat-Sen2

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Insert

    RcaT-Sen2 toxin
    

    Expression

    Bacterial

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB1-mTurquoise

    Plasmid

    #103972

    Purpose

    Plasmid encoding mTurquoise

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mTurquoise
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available Since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pELlR1

    Plasmid

    #172569

    Purpose

    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LacI-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLlacO-1

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pELlR2

    Plasmid

    #172555

    Purpose

    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LacI-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLlacO-1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid

    #101204

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTn7xTS-mPlum

    Plasmid

    #117392

    Purpose

    Tn7 tagging vector pTn7xTS with mPlum expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    mPlum
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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