30,808 results

1. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

   Plasmid

   #87350

   Purpose

   S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/S355C/C574S/S867C

   Promoter

   T7

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHIS-GB1-BP1

   Plasmid

   #133427

   Purpose

   Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.

   Depositor

   Gerhard Wagner

   Insert

   SREBF1 (SREBF1 Human)
   

   Expression

   Bacterial

   Mutation

   SREBP1a amino acids 17-42 fused to GB1 solubilit…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a- mCFP-kanMX6

   Plasmid

   #105145

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mCFP-kanMX6
   

   Tags

   mCFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX SHIP2-SH2

   Plasmid

   #46493

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHIP2
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 8-131

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ETV1A-c014

   Plasmid

   #53655

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   ETV1A
   

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMCSG7-D16A-NmeCas9

   Plasmid

   #71476

   Purpose

   bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-D16A-NmeCas9
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   D16A

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAB082b

   Plasmid

   #79234

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d3
   

   Expression

   Bacterial

   Promoter

   Ppro1

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. monomer-NN

   Plasmid

   #27965

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TAL-monomer-NN
   

   Expression

   Bacterial

   Available Since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

9. PIP4K2AA

   Plasmid

   #42494

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PIP4K2AA (PIP4K2A Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEN-L1-K2-L2

    Plasmid

    #80684

    Purpose

    Gateway Entry vector containing a temperature-sensitive mouse DHFR

    Depositor

    Nico Dissmeyer

    Article

    Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

    Inserts

    DHFR (Dhfr Mouse)
    DHFR (Dhfr Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    mouse DHFR with N-terminal Ubiquitin

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 4MVT

    Plasmid

    #60101

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIAS3:YTC008-B10:C225910 (PIAS3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. FES

    Plasmid

    #38869

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FES (FES Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. KaiA

    Plasmid

    #42497

    Depositor

    Michael Rust

    Article

    Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

    Insert

    Kai A
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    t7

    Available Since

    Feb. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbB7c-GFP

    Plasmid

    #35359

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbA6k-RFP

    Plasmid

    #35291

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

    Plasmid

    #30184

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

    Expression

    Bacterial

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSrpR-S2

    Plasmid

    #74692

    Purpose

    SrpR-S2 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSS2-SrpR-PSrpR-YFP
    

    Expression

    Bacterial

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1 p37

    Plasmid

    #113500

    Purpose

    Bacterial expression of GST-tagged p37

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS17: pTwinStrep-SUMO-AvTnsD

    Plasmid

    #168150

    Purpose

    AvTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsD
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMS15: pTwinStrep-SUMO-AvTnsB

    Plasmid

    #168148

    Purpose

    AvTnsB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsB
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge