30,535 results

1. pET41b+_Npl4

   Plasmid

   #117108

   Purpose

   Production of recombinant Npl4 in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Npl4 (NPLOC4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAW014-2

   Plasmid

   #85612

   Purpose

   Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   ugtP-gRNA.P395T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169193

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFLP-hyg

   Plasmid

   #87831

   Purpose

   Expresses FLP recombinase with hyg selection marker

   Depositor

   Pep Charusanti

   Article

   Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

   Insert

   hyg
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   None

   Promoter

   heat-shock inducible

   Available Since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pACλCI-β-flap (831-1057)

   Plasmid

   #53733

   Purpose

   Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   λCI-β-flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac UV5

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBRα-β flap (831-1057)

   Plasmid

   #53734

   Purpose

   Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   alpha NTD-Beta flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. plRL58

   Plasmid

   #166886

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET His6 GST TEV LIC cloning vector (2G-T)

   Plasmid

   #29707

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Use

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available Since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSFDuet1_CylLL_CylM

    Plasmid

    #208759

    Purpose

    Expresses modified cytolysin L (mCylLL) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLL
    CylLM
    

    Use

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNP1 eforRed trigger

    Plasmid

    #107357

    Purpose

    Trigger sequence for pNP1 eforRed toehold switch

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold trigger 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB064d

    Plasmid

    #79197

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ppro1

    Available Since

    Dec. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACYCDuet-1:ChrA

    Plasmid

    #225079

    Purpose

    Expresses precursor peptide ChrA in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAT13araE

    Plasmid

    #18987

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    araE (araE E.coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19 PKC alpha

    Plasmid

    #8429

    Depositor

    Frederic Mushinski

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Use

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Promoter

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge