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pGT-S2

Plasmid

#122583

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-B2

Plasmid

#122580

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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ADA

Plasmid

#39020

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ADA (ADA Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
TGT-His

Plasmid

#138201

Purpose

Expresses E. Coli TGT with a C-terminal His Tag

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Insert

E Coli tRNA Guanine Transglycosylase-His

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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A24M04 mIgM heavy chain-pFEEKW

Plasmid

#234809

Purpose

Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

Depositor

Susan Pierce

Article

Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

A24M04 membrane IgM heavy chain (IGHM Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EEK promoter

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023

Plasmid

#90324

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBbB5c-GFP

Plasmid

#35353

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdMCP stdHalo

Plasmid

#104999

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

stdMCP-stdHalo

Use

Lentiviral

Tags

HA tag, NLS, Two synonymous copies of the Halo-ta…

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Non tagged Avidin

Plasmid

#214835

Purpose

This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Non tagged Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

lac

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCB’

Plasmid

#162703

Purpose

pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

5513T>G (tetR E37A); 7758G>T (second tet op…

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

HIS-tag

Expression

Bacterial

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p1A

Plasmid

#162692

Purpose

Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLC291

Plasmid

#44448

Depositor

Christopher Marx

Article

Chubiz et al BMC Res Notes. 2013 May 6;6:183. doi: 10

Type

Empty backbone

Use

Rk2, pmb1

Expression

Bacterial

Promoter

P_R (Phage 16-3)

Available Since

Aug. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pJL1

Plasmid

#69496

Purpose

pJL1 expression vector with sfGFP

Depositor

Michael Jewett

Article

Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

Insert

Super folder GFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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