32,686 results
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Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
Tagscp10GFPExpressionBacterial and MammalianPromoterCMV promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rab1A(S20C) CL
Plasmid#224272PurposeProtein purification of Rab1A (S20C)DepositorAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLY071f
Plasmid#227942PurposeSICLOPPS precursor for cyclo-CRCVFSGDepositorInsertEvolved Ssp SICLOPPS-CRCVFSG
ExpressionBacterialMutationEvolved Ssp split inteins with multiple mutations…Available SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
T. aq RNA Polymerase
Plasmid#215349PurposeExpress T. aq RNA Polymerase subunits in E. coli cellsDepositorInsertRNA polymerase
Tags6x HisExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-α-COPI-WD40
Plasmid#208341PurposeBacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40DepositorInsertN-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
TagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISEc41_reRNA
Plasmid#205341PurposeBacterial expression of ISEc41 TnpB and its reRNADepositorInsertISEc41 TnpB
ExpressionBacterialAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISEc46_reRNA
Plasmid#205343PurposeBacterial expression of ISEc46 TnpB and its reRNADepositorInsertISEc46 TnpB
ExpressionBacterialAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISNsp2_reRNA
Plasmid#205350PurposeBacterial expression of ISNsp2 TnpB and its reRNADepositorInsertISNsp2 TnpB
ExpressionBacterialAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SKAP2-1/1
Plasmid#91447PurposeProtein expression and purification of human SH3 domain construct SKAP2-1/1DepositorInsertSKAP2-1/1 (SKAP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_mmHUR-Halo
Plasmid#175311PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-LANA
Plasmid#157801PurposeExpression of mEGFP and LANA peptide fusion proteinDepositorInsertmEGFP-LANA
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-mpx-cxcr4b whim-gfp
Plasmid#41089DepositorInsertCXCR4b-WHIM (cxcr4b Zebrafish)
TagsGFPExpressionBacterialMutationdeleted last 19 amino acids of cxcr4bPromotermpxAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S1
Plasmid#74691PurposeSrpR-S1 gateDepositorInsertPTac-RBSS1-SrpR-PSrpR-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
ExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RL015A
Plasmid#42134DepositorInsertsfGFP-mCherry
ExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only