30,807 results

1. 41 pCOLI_K_NoTag

   Plasmid

   #160265

   Purpose

   Untagged Kan resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPDS1-mEGFP-IRES-puro2b

   Plasmid

   #26464

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   pPDS1 (PTTG1 Human)
   

   Tags

   mEGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-ChmN

   Plasmid

   #221348

   Purpose

   Express ChmN in E. coli

   Depositor

   Emily Balskus

   Article

   Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

   Insert

   chmN
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pxyl-zapT-mScarlet-I

   Plasmid

   #207249

   Purpose

   Integrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoter

   Depositor

   Suliana Manley

   Article

   Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

   Insert

   ZapT-mScarlet-I
   

   Tags

   mScarlet-I

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1 IntS6 AA 1035-1284

   Plasmid

   #196904

   Purpose

   Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 6 (IntS6 Fly)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

   Plasmid

   #187114

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSG00000101199 (ARFGAP1 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGAT3

   Plasmid

   #112589

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His-GST

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAT4

   Plasmid

   #112585

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSV2G_PflaB-mEGFPBb

    Plasmid

    #118234

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven mEGFP
    

    Expression

    Bacterial

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTKIP-cat

    Plasmid

    #41065

    Depositor

    Edward Cox, Thomas Kuhlman

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Expression

    Bacterial

    Promoter

    lacIq

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL1-CyPet

    Plasmid

    #102636

    Purpose

    In vitro expression of CyPet from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    CyPet
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEN1-SGC

    Plasmid

    #39127

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Nicola Burgess-Brown

    Type

    Empty backbone

    Tags

    Strep tag and SET1

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. 4IJD

    Plasmid

    #51328

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJD (PRDM9 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pAV124

    Plasmid

    #63190

    Purpose

    pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF-NtXNtS1R1AtR2B2

    Plasmid

    #160880

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Expression

    Bacterial

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbA5a-RFP

    Plasmid

    #35280

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGG004

    Plasmid

    #113979

    Purpose

    Vector construction module. Level 1 golden gate cloning site module with LacZ reporter

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Level 1 golden gate cloning site
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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