30,807 results

1. pBluescript goosecoid

   Plasmid

   #16935

   Depositor

   John Postlethwait

   Article

   Thisse et al Dev Biol. 1994 Aug . 164(2):420-9.

   Insert

   goosecoid (gsc Zebrafish)
   

   Expression

   Bacterial

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

2. Grp1-PH pET11a

   Plasmid

   #71377

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pT7-agrBD-I

   Plasmid

   #53439

   Purpose

   T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7-lac

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. TOPO ZeoR

   Plasmid

   #68446

   Purpose

   TOPO construct expressing ZeoR for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Zeocin Resistance
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETSUMO2_Rhodoplanes-bGSDM

   Plasmid

   #212129

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Rhodoplanes bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETSUMO2_Bacteroidetes-bGSDM

   Plasmid

   #212124

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bacteroidetes bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETSUMO2_Runella-bGSDM

   Plasmid

   #212123

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Runella bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETSUMO2_Vitiosangium-bGSDM

   Plasmid

   #212127

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Vitiosangium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETSUMO2_Gillisia-bGSDM

   Plasmid

   #212128

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Gillisia bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETSUMO2_Bradyrhizobium-bGSDM

    Plasmid

    #212126

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Bradyrhizobium bGSDM
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET47b-T7-dAsCas12f

    Plasmid

    #204641

    Purpose

    Expression of an inactive AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    inactive AsCas12f
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D225A

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_GNA14_G-alpha

    Plasmid

    #109914

    Purpose

    Protein expression and purification of GNA14_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA14_G-alpha (GNA14 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. N-BLa 1.1

    Plasmid

    #88994

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.1 fusion protein
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Talin Head

    Plasmid

    #186140

    Purpose

    For expression of recombinant Talin head domain in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    TLN1 (TLN1 Human)
    

    Tags

    His6 (tev cleavage)

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescript-mouse eIF2C3

    Plasmid

    #50355

    Purpose

    Mouse eIF2C3 cDNA clone

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C3 (Ago3 Mouse)
    

    Expression

    Bacterial

    Promoter

    T3

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMP4516

    Plasmid

    #107775

    Purpose

    Expression of ECFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-ECFP in pBBR1-mcs5
    

    Tags

    pLacZ-ECFP

    Expression

    Bacterial

    Promoter

    pLacZ

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MBP-MEK1 S222TAG

    Plasmid

    #68302

    Purpose

    N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-EPN01*-His

    Plasmid

    #84509

    Purpose

    E.coli version of EPN01*

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN01*-His_pET29b
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHD_v2

    Plasmid

    #32183

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer HD
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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