32,686 results
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Plasmid#87309PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human MIZ1 wild type
Plasmid#74161Purposebacterial expression of MIZ1DepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-PLY
Plasmid#160537PurposeExpression of inserted PLY variantDepositorInsertPLY-WT
TagsHis-TagExpressionBacterialAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC155
Plasmid#202274PurposeLevel 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent markerDepositorInsertbc182
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC114
Plasmid#202276PurposeLevel 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc141
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3a-DJ1
Plasmid#51489PurposeInducible expression of human DJ1 in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1
Plasmid#37217DepositorInserteIF1
ExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 D321N
Plasmid#53202PurposeExpresses GST and HA tagged human ERK2 D321N from bacterial expression vectorDepositorAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFR
Plasmid#187108PurposeExpression of SNAP-ecDHFR fusion in bacteriaDepositorInsertSNAP-ecDHFR
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)
Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
TagsGSTExpressionBacterialPromoterT7Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
tIVR
Plasmid#122663PurposeExpress tIVR protein in E. coliDepositorInserttIVR
TagsHis-tagExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
ExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
DARS_pNIC-Bio3
Plasmid#153048PurposeExpression construct for producing Avi tagged Glycyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS84, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIcaRA-I1
Plasmid#74682PurposeIcaRA-I1 gateDepositorInsertPTac-RBSI1-IcaRA-PIcaR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-UTPase
Plasmid#12667DepositorAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only