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pBetI-E1

Plasmid

#74677

Purpose

BetI-E1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSE1-BetI-PBetI-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMS8: pTns(AvCAST)

Plasmid

#168141

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_Ub

Plasmid

#172134

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET30 GLTP

Plasmid

#170733

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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KaiA

Plasmid

#42497

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai A

Tags

GST

Expression

Bacterial

Promoter

t7

Available Since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pOPS0378

Plasmid

#133229

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBMTBX-1

Plasmid

#26072

Depositor

Ryan Gill

Article

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_266-341

Plasmid

#118821

Purpose

Bacterial expression of hnRNPA2 truncation

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His and TEV

Expression

Bacterial

Mutation

contains amino acids 266-341

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFVTd5

Plasmid

#167520

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-tdKatushka2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSV272-PfMBP-HypDAE

Plasmid

#110296

Purpose

Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase Activating Enzyme

Tags

His6 and Maltose Binding Protein

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Xenopus (Silurana) tropicalis

Plasmid

#75355

Purpose

Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

Depositor

Kalle Gehring

Insert

Sacsin Xenopus (Silurana) tropicalis 87-1376

Tags

His-Tag

Expression

Bacterial

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk1a1

Plasmid

#15743

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pQE80l A-CC-A

Plasmid

#196478

Purpose

Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

A-CC-A

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_ALDOA_Glycolytic

Plasmid

#109854

Purpose

Protein expression and purification of ALDOA_Glycolytic

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ALDOA_Glycolytic (ALDOA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GC83 pComb3xss anti-Gephyrin nanobody

Plasmid

#134721

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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TDRD3 (3PMT)

Plasmid

#28274

Depositor

Cheryl Arrowsmith

Insert

Tudor domain-containing protein 3 (TDRD3 Human)

Tags

His with TEV cleavage

Expression

Bacterial

Mutation

contains Tudor domain, residues 553-611.

Available Since

April 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-2T_PDZ2

Plasmid

#59735

Purpose

expresses PDZ2 domain of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

Insert

zonula occludens-1 (TJP1 Human)

Tags

GST and thrombin clvg

Expression

Bacterial

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only