31,182 results

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pJMP2774

Plasmid

#160667

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAB088i

Plasmid

#79216

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET_scFv_QLuc

Plasmid

#183261

Purpose

Express scFv_QLuc in bacteria cells

Depositor

Huiwang Ai

Article

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Insert

scFv_QLuc

Tags

Histag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1039-1176

Plasmid

#53351

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1039-1176

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-β4 hinge-ear

Plasmid

#196692

Purpose

Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 β4 hinge-ear (534-739) (AP4B1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSFBAD09

Plasmid

#11963

Depositor

Hideo Iwai

Article

Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

Insert

SspDnaEC

Tags

CBD

Expression

Bacterial

Available Since

Sept. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pCoofy16

Plasmid

#43988

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-NusA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pIB167

Plasmid

#90190

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR6k-con-GFPuv

Plasmid

#106387

Purpose

Constitutive LlacO-1 promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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B7-3

Plasmid

#111491

Purpose

Tyr-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Tyr-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLOXp-GUS

Plasmid

#154059

Purpose

Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

beta-glucoronidase (E. coli)

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-R273H-N268D

Plasmid

#35087

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R273H-N268D

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
K401-Micro

Plasmid

#122485

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

His tag MBP Kinesin 1-401 SspB micro

Tags

H6-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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K401-iLid

Plasmid

#122484

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

Kinesin 1-401 iLid His tag

Tags

His6 and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Promoter

T7

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pXDC61-RalF

Plasmid

#21844

Depositor

Howard Shuman

Article

Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

Insert

RalF

Tags

TEM

Expression

Bacterial

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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