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32,457 results

Showing: 10821 - 10840 of 32457 results

  3. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  6. pGEX2TK-Num1-PH

    Plasmid
    #71369
    Purpose
    bacterial expression of PH domain for phosphoinositide binding assay
    Depositor
    Mark Lemmon
    Article
    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.
    Insert
    Num1-PH
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Pleckstrin homology (PH) domain; aa2563-2692
    Promoter
    tac
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pAM5082

    Plasmid
    #85121
    Purpose
    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiB-KaiC-ECFP
    Tags
    ECFP
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85121-plasmid-map-sequence-id-180106

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  11. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pAV123

    Plasmid
    #63189
    Purpose
    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63189-plasmid-map-sequence-id-331731

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  19. pOPS0378

    Plasmid
    #133229
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133229-plasmid-map-sequence-id-276542

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Showing: 10821 - 10840 of 32457 results