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34,239 results

Showing: 10821 - 10840 of 34239 results

  1. pGEX2TK-YAP-WW1-RRK

    Plasmid
    #104306
    Purpose
    bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated
    Depositor
    Marius Sudol
    Insert
    YAP WW1 domain (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…
    Promoter
    tac
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104306-plasmid-map-sequence-id-201230

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  9. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Publication
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  10. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Publication
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  11. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Publication
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  12. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

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  14. pMflT-o4

    Plasmid
    #101312
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Publication
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101312-plasmid-map-sequence-id-198865

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  15. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  17. pEV_S

    Plasmid
    #100266
    Purpose
    pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli
    Depositor
    Timothy Lu
    Publication
    Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.
    Inserts
    entD (entD Escherichia coli)
    entC
    entE
    entB
    entA
    vibH
    vibF
    cos
    Use
    Synthetic Biology; Expression, pbr322
    Expression
    Bacterial
    Available since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100266-plasmid-map-sequence-id-193235

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  18. 5VC9 (CXXC4)

    Plasmid
    #101263
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC4 (CXXC4 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101263-plasmid-map-sequence-id-197652

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  20. ZMYND8 (PRKCBP1A-c097)

    Plasmid
    #98244
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRKCBP1A (ZMYND8 Human)
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98244-plasmid-map-sequence-id-193422

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Showing: 10821 - 10840 of 34239 results