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MBP-TEV-VMA Model vector

Plasmid

#86590

Purpose

This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

Depositor

Sharon Rozovsky

Publication

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

VMA intein

Tags

His6-MBP

Expression

Bacterial

Mutation

Original SapI site from pMal was mutated out

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLacI241-Wasabi

Plasmid

#86102

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI A241T

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A241T

Promoter

T7A1O4/O3 and pLacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-PATagRFP-KanMX6

Plasmid

#87027

Purpose

Tag S. pombe genes in C terminal with PATagRFP

Depositor

Julien Berro

Insert

PATagRFP

Expression

Bacterial and Yeast

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Publication

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-TEM1(S70A,D179G)/csTEM1

Plasmid

#81163

Purpose

pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

Depositor

Timothy Whitehead

Publication

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

Codon swapped TEM-1 from H26 to W290 and His6x

Expression

Bacterial

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

lac promoter

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGEN6-Ubc5 Sc

Plasmid

#83234

Purpose

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

Depositor

Gali Prag

Publication

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Inserts

Ubiqutin (UBC Human)
Ubc 5 (UBC5 Budding Yeast)
Uba 1

Tags

His

Expression

Bacterial

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_3

Plasmid

#68809

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Publication

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-09

Plasmid

#79924

Purpose

Palm3-12GS-I3-01-myc-Late1

Depositor

Neil King

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-09

Expression

Bacterial and Mammalian

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pRAF-MutH

Plasmid

#86609

Purpose

E. coli MutH expression plasmid

Depositor

Richard Fishel

Publication

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutH

Tags

his-tag and ald-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GST-Laforin

Plasmid

#84673

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Publication

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b(+)-SPN+IFS

Plasmid

#83372

Purpose

Expresses SPN and IFS complex in E. coli cell

Depositor

SeWon Suh

Publication

Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

Inserts

SPN
IFS

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Bacterial

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pCFJ889

Plasmid

#84826

Purpose

Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selection

Depositor

Andrew Fire

Publication

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

smu-1 (smu-1 Nematode)

Tags

GFP

Expression

Bacterial and Worm

Promoter

Psmu-1

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pWURA Cas1

Plasmid

#80089

Purpose

Encodes Cas1

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Insert

Cas1 (cas1 )

Expression

Bacterial

Promoter

T7

Available since

Dec. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Publication

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJPC12-ΔM13-mCherry-P/PM,4A5C6G7G-GFP

Plasmid

#80914

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P4A5C6G7G and PM,4A5C6G7G

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLITMUS-rpoN-cI5G6G,P-J23106-geneIII

Plasmid

#80861

Purpose

Phagemid (encodes evolved cI5G6G,P and M13 gene III)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLITMUS-rpoN-cI5C6A-J23106-geneIII

Plasmid

#80905

Purpose

Phagemid (encodes cI5C6A and M13 gene III)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP

Plasmid

#80910

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5C6A and PM,5C6A

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3R112C/T180CBM3

Plasmid

#85090

Purpose

Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

Depositor

Teruyuki Nagamune

Publication

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The R112C/T180C variant of PCNA3 fused to P450 BM3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

Promoter

T7 promoter

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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