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pBTK1003 (pSL2)

Plasmid

#190998

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR1

Plasmid

#136361

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR2

Plasmid

#136362

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBbB11a-GFP

Plasmid

#126114

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23150

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNIDR

Plasmid

#157868

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNIDR

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBMTBX-1

Plasmid

#26072

Depositor

Ryan Gill

Article

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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GST-FBL

Plasmid

#179245

Purpose

protein purification in E. coli

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

FBL (FBL Human)

Tags

GST

Expression

Bacterial

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET MBP mOrange LIC cloning vector (MBP-mOrange)

Plasmid

#29748

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pQE80l A-CC-A

Plasmid

#196478

Purpose

Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

A-CC-A

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSKDuet26

Plasmid

#41689

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

Tvo VMA intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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Reb_65 aka PAS626

Plasmid

#71231

Purpose

Reb_1 with RebA(Y82A)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, Y82A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(504)

Plasmid

#44372

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAB061i2

Plasmid

#79229

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSM015

Plasmid

#128289

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-cfp-parB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG216

Plasmid

#114002

Purpose

Level 2 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pHsSR beta deltaN Flag

Plasmid

#13731

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRPRB Human)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

expresses residues 59-271 of Homo sapiens SRβ wit…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMVV098

Plasmid

#215304

Purpose

Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PPhIF

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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