30,803 results

1. M26I DJ-1 pET15b

   Plasmid

   #60677

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I

   Promoter

   T7

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBbdCas9S(-10TC)_Psyn-sgRNA500

   Plasmid

   #149583

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-Epsin 2

   Plasmid

   #22241

   Depositor

   Pietro De Camilli

   Article

   Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

   Insert

   Epsin 2 (EPN2 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

4. R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

   Plasmid

   #159564

   Purpose

   E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D (KRAS Human)
   

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Mutation

   G13D

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. R701-X53-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

   Plasmid

   #159561

   Purpose

   E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C (KRAS Human)
   

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Mutation

   G12C

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a(+)_C.jejuni m23 peptidase

   Plasmid

   #83297

   Purpose

   Expresses m23 peptidase from Campylobacter jejuni

   Depositor

   SeWon Suh

   Insert

   ERS445056_01343
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-20

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_LYN-1/1

   Plasmid

   #91536

   Purpose

   Protein expression and purification of human SH3 domain construct LYN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   LYN-1/1 (LYN Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCoofy61

   Plasmid

   #122013

   Purpose

   E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

   Depositor

   Peggy Stolt-Bergner, Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-eGFP

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQLinkHD-SYNZIP3

   Plasmid

   #80659

   Purpose

   Encodes the synthetic coiled coil peptide SYNZIP3

   Depositor

   Amy Keating

   Article

   Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

   Insert

   SYNZIP3
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMUT2-pCaf-mCherry

    Plasmid

    #192860

    Purpose

    pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Nanobody-CadC fusion protein and cognate promoter inducible by caffeine
    

    Expression

    Bacterial

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT3035

    Plasmid

    #122543

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_lmn-1::CemOrange2 (lmn-1 Nematode, Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-UPRT

    Plasmid

    #69310

    Purpose

    expresses UPRT cDNA

    Depositor

    Chris Doe

    Article

    Gay et al Nat Protoc. 2014 Feb;9(2):410-20. doi: 1

    Insert

    UPRT cDNA
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLITMUS-rpoN-cI5C6A,P-J23106-geneIII

    Plasmid

    #80860

    Purpose

    Phagemid (encodes evolved cI5C6A,P and M13 gene III)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    cI5C6A,P
    

    Expression

    Bacterial

    Promoter

    BBa_J23106 and rpoN

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbA19a-RFP

    Plasmid

    #126101

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23105

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOSV804

    Plasmid

    #126597

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. YWHAH

    Plasmid

    #38814

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAH (Ywhah Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBAD/HisB (TIREVOL)-mEos3.2

    Plasmid

    #189720

    Purpose

    titratable expression of mEos3.2 in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mEos3.2
    

    Tags

    His-T7-EK

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX2T-TUBG1

    Plasmid

    #171967

    Purpose

    pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

    Depositor

    Maria Alvarado-Kristensson

    Article

    Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

    Insert

    TUBG1 (TUBG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R275H (Please see depositor comments)

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. sfTq2-PBP5

    Plasmid

    #117959

    Purpose

    Periplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5)

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    sfTq2-PBP5 (dacA )
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM33_Nsp3b_ADRP

    Plasmid

    #156468

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3b_ADRP (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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