33,483 results

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pXRkMX

Plasmid

#63147

Purpose

Kan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Publication

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pXRH3

Plasmid

#63145

Purpose

His3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Publication

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-ITPA

Plasmid

#60824

Purpose

to purify human ITPA from bacteria

Depositor

Youri Pavlov

Publication

Porta et al Acta Crystallogr Sect F Struct Biol Crys

Insert

ITPA (ITPA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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4TVR

Plasmid

#61952

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

UBH14 (UHRF2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAND_Int2

Plasmid

#60590

Purpose

A transcriptional AND circuit constructed by layering NOR and NOT gates

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Int2

Expression

Bacterial

Promoter

PPhlF

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAND_yfp

Plasmid

#60592

Purpose

used with pSpec to generate an AND gate without memory

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_yfp

Expression

Bacterial

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET23a_mod_RBPMS2_FL

Plasmid

#59394

Purpose

Plasmid for bacterial overexpression of RBPMS2

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

6xHis, FLAG, HA, and T7

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Publication

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pSARSF53-110

Plasmid

#61813

Purpose

TvoVMAN11 intein

Depositor

Hideo Iwai

Publication

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAN11 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSABAD14-98

Plasmid

#61827

Purpose

TvoVMAdN11 intein

Depositor

Hideo Iwai

Publication

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdN11 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Publication

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET23a_mod_RBPMS_FL

Plasmid

#59393

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

6xHis, FLAG, and HA

Expression

Bacterial

Promoter

T7

Available since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAtLCYbSK

Plasmid

#53588

Purpose

Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

Insert

lcyB (LYC Mustard Weed)

Expression

Bacterial

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Hs_MSI2_RBD

Plasmid

#61113

Purpose

Express His tagged human MSI2 RNA binding domain

Depositor

Sean Ryder

Insert

MSI2 RNA binding domain (MSI2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-1b

Plasmid

#61327

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Publication

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MBP-tag

Expression

Bacterial

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pTHSSd_42

Plasmid

#59965

Purpose

beta core fragment expression

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

beta core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_10

Plasmid

#59950

Purpose

PK1F GFP reporter

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PK1F

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCri-4a

Plasmid

#61314

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Publication

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET:ACAT:GFP

Plasmid

#60740

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET:GCAT:GFP

Plasmid

#60736

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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