30,736 results

1. pCasKP-hph

   Plasmid

   #117232

   Purpose

   Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-SpyCatcher

   Plasmid

   #35044

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28 MBP_PrA/G-H6

   Plasmid

   #176077

   Purpose

   Express His6 tagged MBP_PrA/G in Escherichia coli

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   MBP PrA/G-H6
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP955

   Plasmid

   #104164

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMal-T-Avi-His/BirA

   Plasmid

   #102962

   Purpose

   Vector for in vivo biotinylation of a fusion protein in bacteria.

   Depositor

   Tonia Rex

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6TEV-UbG76C

   Plasmid

   #75299

   Purpose

   Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   human Ubiquitin mutant G76 to C
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Glycine 76 to Cystine

   Promoter

   T7

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 TEV LIC cloning vector (2B-T)

   Plasmid

   #29666

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a MDHC_YEAST

   Plasmid

   #204232

   Purpose

   Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHC_YEAST (MDH2 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKB225

   Plasmid

   #170015

   Purpose

   Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Peptide display protein with no C-terminal peptide
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Promoter

   Ptac and PvirK

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7C-HSP90AA1

    Plasmid

    #177658

    Purpose

    Recombinant protein synthesis of human HSP90AA1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AA1 (HSP90AA1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Tags

    strep tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLp_3050sNuc

    Plasmid

    #122030

    Purpose

    Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

    Depositor

    Vincent Eijsink, Geir Mathiesen

    Article

    Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

    Insert

    signal peptide Lp_3050
    

    Expression

    Bacterial

    Promoter

    sppA

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBWB419

    Plasmid

    #140637

    Purpose

    RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTHSSe_48

    Plasmid

    #109246

    Purpose

    pSC101 var6 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-alpha-synuclein-BiFC

    Plasmid

    #87855

    Purpose

    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    alpha-synuclein-VC155
    alpha-synuclein-VN154
    

    Tags

    C-terminal fragment of mVenus BiFC protein and N-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG012 (DRT type 4)

    Plasmid

    #157890

    Purpose

    Expresses the DRT type 4 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 4
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLG013 (DRT type 5)

    Plasmid

    #157891

    Purpose

    Expresses the DRT type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 5
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE-Tn5-132

    Plasmid

    #118518

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmal_c5x_RNAse_Inhib

    Plasmid

    #153314

    Purpose

    Bacterial expression of an RNase inhibitor

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    RNAse Inhibitor
    

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSR47S Cm

    Plasmid

    #41892

    Depositor

    Howard Steinman

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb

    Expression

    Bacterial

    Available Since

    May 1, 2013

    Availability

    Academic Institutions and Nonprofits only