30,744 results

1. pBad-HisB-GGvT

   Plasmid

   #87362

   Purpose

   Expresses GGvT, a tandem homodimer green fluorescent protein

   Depositor

   Robert Campbell

   Article

   Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

   Insert

   GGvT
   

   Tags

   His-Tag

   Expression

   Bacterial

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET14b_tev_PDZ2

   Plasmid

   #59734

   Purpose

   expresses PDZ2 domain of human ZO-1 in bacteria

   Depositor

   Alan Fanning

   Article

   Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

   Insert

   zonula occludens-1 PDZ2 (TJP1 Human)
   

   Tags

   HIS and TEV

   Expression

   Bacterial

   Available Since

   Sept. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET-(neg27)GFP-GGS9-Cre-6xHis

   Plasmid

   #89253

   Purpose

   Expresses GFP with -27 theoretical charge fused to Cre in E.Coli

   Depositor

   David Liu

   Insert

   (neg27)GFP-GGS9-Cre-6xHis
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH522

   Plasmid

   #102675

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Expression

   Bacterial

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOSV808

   Plasmid

   #126601

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1217

   Plasmid

   #119258

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC010 LshCas13a locus with nontargeting spacer

   Plasmid

   #91901

   Purpose

   LshCas13a locus into pACYC184 with nontargeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LshCas13a locus with non-targeting spacer
   

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCDF-NtXNtT2R1AtR2NtB2

   Plasmid

   #160886

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   PT7-Nt-rbcS-T2
   

   Expression

   Bacterial

   Promoter

   rbcS-T2: T7, chaperones: T9

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28CLY6

   Plasmid

   #21765

   Depositor

   Maarten Merkx

   Article

   Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

   Insert

   ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pMalAnPL

    Plasmid

    #67281

    Purpose

    Expresses MBP-Anacystis nidulans photolyase in E. coli

    Depositor

    Aziz Sancar

    Insert

    photolyase Anacystis nidulans
    

    Tags

    maltose binding protein

    Expression

    Bacterial

    Mutation

    none known

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ2077.2

    Plasmid

    #140666

    Purpose

    Controller plasmid for TS circuit and cL and cR controls

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    Controller plasmid for TS circuit and cL and cR controls
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PAHA

    Plasmid

    #74663

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PKU1 (PAH Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pHAGE2-TetOminiCMV-SM

    Plasmid

    #136578

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-E2A-cMyc (Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCYP01

    Plasmid

    #212834

    Purpose

    Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Expression

    Bacterial

    Promoter

    PcymRC

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-MEK1 S218TAG

    Plasmid

    #68301

    Purpose

    N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDuet1-WT-LanCL2-C-Ter-His

    Plasmid

    #154186

    Purpose

    To express LanCL2 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBM19-sd4U

    Plasmid

    #179692

    Purpose

    Bacterial expression of base editor

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd4U] UGI-TALE4-DddA-G1397-C (T1413I)
    

    Expression

    Bacterial

    Mutation

    T1413I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NME3

    Plasmid

    #25536

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NME3 (NME3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pDSG578

    Plasmid

    #214215

    Purpose

    integrase plasmid

    Depositor

    Uri Alon

    Article

    Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

    Insert

    PhlF, A118
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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