30,736 results

1. pOPINK-OTUD1 (OTU, aa 287-437)

   Plasmid

   #61407

   Purpose

   Expresses human OTUD1 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286 and aa 438-481.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-soNadV

   Plasmid

   #83363

   Purpose

   Expresses nadV from Shewanella oneidensis in Escherichia coli

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Insert

   nadV
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC-0

   Plasmid

   #124421

   Purpose

   Full MCS (step 2)

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Endophilin SH3 domains

   Plasmid

   #47401

   Depositor

   Peter McPherson

   Article

   Micheva et al J Biol Chem. 1997 Oct 24;272(43):27239-4

   Insert

   Endophilin
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   SH3 domain (aa 292-352)

   Promoter

   tac

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

5. MSP577

   Plasmid

   #98801

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
   

   Expression

   Bacterial

   Mutation

   I138T, N171D, N176T

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7pr-His6-MBP-TEV-dCas9-BirA*

   Plasmid

   #159994

   Purpose

   Bacterial expression of dCas9-BirA*

   Depositor

   Jacob Corn

   Article

   Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

   Insert

   dCas9-BirA*
   

   Tags

   6X-His, MBP, 3X-FLAG, NLS

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   T7

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Citrine-pBAD

   Plasmid

   #54772

   Purpose

   Localization: Protein Expression Vector , Excitation: 516, Emission: 529

   Depositor

   Robert Campbell, Roger Tsien, Oliver Griesbeck, Michael Davidson

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Type

   Empty backbone

   Tags

   6xHis and Citrine

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pHH0103_MYO15A-1/2

   Plasmid

   #91491

   Purpose

   Protein expression and purification of human SH3 domain construct MYO15A-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   MYO15A-1/2 (MYO15A Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAKgfp2.2

   Plasmid

   #14079

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   lacIq repressor gene
   

   Expression

   Bacterial

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pQTEV-COPE

    Plasmid

    #31585

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    coatomer protein complex, subunit epsilon (COPE Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-T5-chi28TAG

    Plasmid

    #188984

    Purpose

    Express chimadanin with TAG at 28 position

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    Chimadanin with TAG at 28 position
    

    Expression

    Bacterial

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-mcpGFP

    Plasmid

    #47442

    Purpose

    contains modified circularly permutated EGFP (mcpGFP)

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    mcpGFP
    

    Expression

    Bacterial

    Mutation

    M154K, V164A, S176G, D181Y, T204V, A207K, V190I

    Available Since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pXL-BACII-attPGAL4LWL

    Plasmid

    #26260

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZH518

    Plasmid

    #102671

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK2_HUMAN_D0

    Plasmid

    #79726

    Purpose

    This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDK2 (CDK2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pExp-DsbA

    Plasmid

    #112562

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-DsbA(AA20-208) and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPt_Cp

    Plasmid

    #206855

    Purpose

    A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. B1 Integrin

    Plasmid

    #186137

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Tags

    MBP and untranslated His6 after stop codon

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG8H-EWS_1-264

    Plasmid

    #180467

    Purpose

    Expresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tag

    Depositor

    David Libich

    Article

    Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted 265-656

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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