30,736 results

1. pET SUMO-FL-syt1

   Plasmid

   #213628

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 (Syt1 Rat)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1161

   Plasmid

   #119251

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a-GFP-Patronin CC 868–1457

   Plasmid

   #59053

   Purpose

   for E. coli expression of Patronin CC truncation

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pDM_noro_A2_lacZ

   Plasmid

   #118820

   Purpose

   T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch A2
   

   Expression

   Bacterial

   Available Since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKCyGe2

   Plasmid

   #167515

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymRAM-GmR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCymRC

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFTK043

   Plasmid

   #171315

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   eGFP fluorescent reporter
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. DinoIII-arrO-N

   Plasmid

   #128143

   Purpose

   Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

   Depositor

   Senjie Lin

   Article

   Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

   Insert

   Rifampin ADP-ribosylating transferase + potential promoter
   

   Expression

   Bacterial

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PTPN18

   Plasmid

   #39168

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPN18 (PTPN18 Human)
   

   Tags

   GST-PreScission

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCHERRY8

    Plasmid

    #24663

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTHS

    Plasmid

    #166938

    Purpose

    Toehold switch multi-level gene expression controller (MLC)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Genetic circuit expressing GFP- Toehold switch
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PTAC

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-SF3B1-NTerm

    Plasmid

    #97425

    Purpose

    Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

    Depositor

    Alex Minella

    Article

    Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

    Insert

    SF3B1-N-term (AA1-500)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Silent mutation at nt876, aa289 (AGG to AGA)

    Promoter

    tac

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNBU2_erm_P1T_DP-B1

    Plasmid

    #90319

    Purpose

    Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFAB3689

    Plasmid

    #47834

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 3 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a+GFP-CAMSAP1 CKK

    Plasmid

    #59031

    Purpose

    expresses CKK domain of CAMSAP1 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP1 CKK domain (CAMSAP1 Human)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pRSET-TEV-12His

    Plasmid

    #137032

    Purpose

    empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Type

    Empty backbone

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. sCFP in pBSDONR P4r-P2

    Plasmid

    #121530

    Purpose

    For making C-terminal sCFP fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    sCFP
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CHIKV-nsP3-c001

    Plasmid

    #173086

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    N/A
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A1334-T1493, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MDO2-c003

    Plasmid

    #173105

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD2 (MACROD2 Human)
    

    Expression

    Bacterial

    Mutation

    K7-D243

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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