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pRFP

Plasmid

#26924

Depositor

Ron Weiss

Article

Basu et al Nature. 2005 Apr 28. 434(7037):1130-4.

Insert

LacI, HcRed

Expression

Bacterial

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pETDuet-TFIIF

Plasmid

#171082

Purpose

Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Inserts

RAP30 (GTF2F2 Human)
RAP74 (GTF2F1 Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCURE

Plasmid

#23013

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

I-CreI homing endonuclease

Expression

Bacterial

Mutation

I-CreI expression is controlled by the arabinose …

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pAB076i7

Plasmid

#79201

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a_MBP_RBFOX2

Plasmid

#224292

Purpose

MBP-RBFOX2 protein purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 (RBFOX2 Human)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP

Plasmid

#166443

Purpose

Bacterial expression of 6x His tagged YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCerulean-PA-Rac1

Plasmid

#22030

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His and mCerulean

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pMAL-PUM1

Plasmid

#120384

Purpose

Bacterial expression of MBP-Pumillio1 fusion protein

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM1 (PUM1 Human)

Tags

6xHIS and MBP

Expression

Bacterial

Promoter

LacI

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Alpha1

Plasmid

#118044

Purpose

GoldenBraid2.0 Alpha 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.1642

Plasmid

#108535

Purpose

CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CinRAM + PCin-YFP

Expression

Bacterial

Mutation

CinR: S72C V80I G114D G115D R137H K220R A224T *24…

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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