32,453 results
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Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTmpKm
Plasmid#176227PurposeA template plasmid for amplification of the cI-hok-neo cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, neo
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
ExpressionBacterialPromoterAmpR, CAT, pBAD, and pTrcAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-BCCP-PupE
Plasmid#113403PurposeExpresses BCCP-PupE in bacteriaDepositorInsertBCCP-PupE
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MP28445 – pFUSE2ss-aRD114_CLIg-mIgK
Plasmid#110556PurposepFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.DepositorInsertanit-RD114 light chain
TagsmKappaExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trxAChEwt
Plasmid#83916PurposeEncodes an E. coli codon optimized version of wild type human acetylcholinesteraseDepositorInsertShort form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
TagsHis, S-tag, and TRXExpressionBacterialMutationcodon optimised for E.coli expressionPromoterT7 promoterAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only