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We narrowed to 149 results for: staphylococcus

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Showing: 101 - 120 of 149 results

  1. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

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  2. pJBEI-6410

    Plasmid
    #47049
    Purpose
    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Mutation
    R364S mutation in MK
    Promoter
    PlacUV5
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47049-plasmid-map-sequence-id-496830

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  3. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  10. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  11. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  12. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  13. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  14. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  16. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available Since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    75145-plasmid-map-sequence-id-466935

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  19. eSrtA(4S-9) pET29b

    Plasmid
    #75146
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    N98D S102C A104V A118T F122A K134R F144L I182V E1…
    Promoter
    T7
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    75146-plasmid-map-sequence-id-142065

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Showing: 101 - 120 of 149 results