We narrowed to 138 results for: staphylococcus

1. pET28a Halotag-CNA35

   Plasmid

   #131128

   Purpose

   Halotag-CNA35 peptide for Collagen labelling

   Depositor

   Samantha Stehbens

   Article

   Ju et al bioRxiv 2022

   Insert

   Halotag-CNA35
   

   Use

   Tags

   Halotag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAureus-TnCAM

   Plasmid

   #199807

   Purpose

   vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

   Depositor

   Steve Salipante

   Article

   Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

   Insert

   transposon cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30b-5M SrtA with TEV site

   Plasmid

   #86962

   Purpose

   Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

   Depositor

   Ron Bose

   Article

   Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

   Insert

   S.aureus SrtA 5M
   

   Use

   Tags

   6x Histidine tag and TEV site

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPSA5Cas9syn2

   Plasmid

   #206963

   Purpose

   S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

   Depositor

   Daijie Chen

   Article

   Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPSA5Cas9syn

   Plasmid

   #206965

   Purpose

   S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

   Depositor

   Daijie Chen

   Article

   Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH1-SpSrtA

   Plasmid

   #64987

   Purpose

   Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   S. pyogenes sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-81

   Promoter

   T7 promoter

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIntegrase_8

   Plasmid

   #60579

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGDP3 apmA

    Plasmid

    #112881

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    apmA (apmA Staphylococcus aureus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGDP4 ermC

    Plasmid

    #112884

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    ermC (ermM Staphylococcus epidermis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Use

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLQ-KO-tgt50

    Plasmid

    #126578

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIntegrase_12

    Plasmid

    #60583

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. LacD_WT_pET21a

    Plasmid

    #29785

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. LacD_L165E_pET21a

    Plasmid

    #29786

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. LacD_L275S_pET21a

    Plasmid

    #29787

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L275S

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

19. LacD_L165E/L275S_pET21a

    Plasmid

    #29788

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E and L275S

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pECTO

    Plasmid

    #67683

    Purpose

    pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureus

    Depositor

    Philippe Bouloc

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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