30,258 results

1. pTlpA36-Wasabi

   Plasmid

   #86115

   Purpose

   mWasabi reporter gene gated by TlpA36

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA36
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A, P60L, D135V, K187R, K202I, L208Q

   Promoter

   pTlpA and pTlpA, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas9-CR4

   Plasmid

   #62655

   Purpose

   Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   Cas9 nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 6xHis-SNAP-PI(4)P Probe

   Plasmid

   #211509

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   SidC (P4C)
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCJ189

   Plasmid

   #162666

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-3c-rhVTN-NC

   Plasmid

   #30226

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N and C terminus cleavages (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478, 20-62

   Promoter

   Available since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK1043

   Plasmid

   #8835

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   tobacco etch virus protease, catalytic domain
   

   Use

   Tags

   MBP and polyarginine

   Expression

   Bacterial

   Mutation

   Autolysis-resistant S219V mutant

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p406ADH1

   Plasmid

   #15974

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   ADH1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pColE1-ISceI

   Plasmid

   #179578

   Purpose

   Expresses I-SceI from an arabinose promoter

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   I-SceI (I-SceI Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-his-3C_Tau352

   Plasmid

   #100092

   Purpose

   Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

   Depositor

   Nikolai Sluchanko

   Article

   Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

   Insert

   Fetal microtubule-associated protein tau (MAPT Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   wild type sequence

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK172-6xHis-0N4R-P301S-tau-HiBiT

    Plasmid

    #187835

    Purpose

    Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    6XHis-0N4R-P301S-tau-HiBiT
    

    Use

    Tags

    6xHistidine and HiBiT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGGF_RT_FAST_Tq2CFP

    Plasmid

    #136981

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Insert

    seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDule2 - Mj Acd A9

    Plasmid

    #197652

    Purpose

    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

    Depositor

    Ryan Mehl

    Article

    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

    Inserts

    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32A, L65D, F108L, Q109E, D158S, L162T

    Promoter

    GlnS and lpp

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET22-HT/plsA

    Plasmid

    #73625

    Purpose

    Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

    Depositor

    James Williamson, T. J. Kappock

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMyrPalm_mEGFP

    Plasmid

    #21037

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pMyrPalm
    

    Use

    Tags

    mEPFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pRSFDuet-1_DNAseI

    Plasmid

    #182408

    Purpose

    Bacterial expression of DNaseI

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11b-HA2

    Plasmid

    #165992

    Purpose

    Influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    HA2
    

    Use

    Tags

    Foldon and HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLG163

    Plasmid

    #124365

    Purpose

    IPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pspank

    Available since

    July 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD_dGFPmut3-AAV

    Plasmid

    #187379

    Purpose

    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

    Depositor

    Roi Avraham

    Insert

    dGFPmut3_AAV
    

    Use

    Tags

    AAV

    Expression

    Bacterial

    Mutation

    C-terminal AAV tail

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge