29,920 results

1. pET28_6xHis-PARP1-Zn2

   Plasmid

   #174805

   Purpose

   Bacterial expression of isolated PARP1 Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn2 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_6xHis-PARP1-Zn3

   Plasmid

   #174806

   Purpose

   Bacterial expression of isolated PARP1 Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn3 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His-Tev-UbE1

   Plasmid

   #186804

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE1 (UBA1 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGRG25

   Plasmid

   #16665

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 . 6():39.

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV ie enhancer/promoter

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pET21a-Streptavidin-Alive

   Plasmid

   #20860

   Purpose

   Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Alive
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Wt gene

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human_TFAM_NoMTS_pET28

   Plasmid

   #34705

   Depositor

   David Chan

   Article

   Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Encodes residues 43–246, corresponding to full-le…

   Promoter

   T7

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWM_12x601_25bpLinker

   Plasmid

   #157788

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_25bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-29b(+)_NSP4ss_SerM_NucA-His

   Plasmid

   #218190

   Purpose

   Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   His and NSP4 signal secretion peptide

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDuet_huCAD_ICADL (TevS)

    Plasmid

    #100098

    Purpose

    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two caspase cleavage sites (DETD-117 and DAVD-224…

    Promoter

    T7

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge