30,410 results

1. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Promoter

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. human p53-(1-393)

   Plasmid

   #24859

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Full length.

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRKH3-slpGFP

   Plasmid

   #27168

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. JC Mad-1

   Plasmid

   #25626

   Depositor

   Peter Howley

   Article

   Howley et al J Virol. 1980 Dec . 36(3):878-82.

   Insert

   JC Virus Mad-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pOPINK-OTUD6B (full-length, aa 1-293)

   Plasmid

   #61418

   Purpose

   Expresses human OTUD6B (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6B (OTUD6B Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SRPK1 (SRPK1A-c100)

   Plasmid

   #98246

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SRPK1A (SRPK1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-mCherry-CNA35

   Plasmid

   #61607

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mCherry

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mCherry and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11a+CYP119

   Plasmid

   #66131

   Purpose

   Expresses the wild type of CYP119

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid

    #128164

    Purpose

    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    

    Use

    Synthetic Biology

    Tags

    FLAG and zif268

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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